Table 2 |
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|
Effect of methanol concentration and induction time on recombinant protein yield |
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|
Methanol concentration and induction time |
||||
|
|
||||
|
0.50% |
1% |
|||
|
|
||||
|
Construct |
48 h |
120 h |
120 h |
|
|
|
||||
|
var2csa 7G8 DBL5 |
Q2000-T2318 |
1.2 mg/L |
0.9 mg/L |
Nt |
|
var2csa IT DBL2-ID2 |
D517-G1229 |
0 |
0 |
0 |
|
var2csa IT DBL3 |
L1221-E1541 |
0.1 mg/L |
0.375 mg/L |
1 mg/L |
|
var2csa 3D7 DBL5 |
N1888-T2291 |
0.21 mg/L |
0.46 mg/L |
Nt |
|
var2csa 7G8 DBL4-DBL5 |
G1578-2318 |
0 |
0.1 mg/L |
Nt |
|
var22 IT DBL3 |
G1180-N1488 |
0 |
nt |
0.75 mg/L |
|
var18 IT DBL3 |
N1247-D1545 |
0 |
nt |
0.125 mg/L |
|
|
||||
|
P. pastoris clones were grown overnight at 0.9 L to build biomass and protein was induced at 0.3 L scale at different durations and methanol concentrations. nt, not tested. |
||||
|
Avril et al. Malaria Journal 2009 8:143 doi:10.1186/1475-2875-8-143 |
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