Figure 1.

Schematic representation of the MSP-1 and MSP-1/MSP-6/MSP-7 complex antigens used in the study. The schematic representation of the MSP-1 protein is shown (A). The MSP-1 precursor protein (i) undergoes proteolytic cleavage into four subunits as shown (ii). The MSP-1₄₂ molecule is further cleaved to MSP-1₃₃ and MSP-1₁₉ (iii). A proposed model (adapted from [23]) demonstrating the interactions of MSP-1 protein with the MSP-6₃₆ and MSP-7₂₂ molecules (B). The two allelic forms of MSP-1₈₃, MSP-1₃₀, MSP-1₃₈ and MSP-1₄₂ (D and F), in addition to MSP-6₃₆, MSP-7₂₂ and MSP-1₁₉, were expressed in E. coli and purified [49,50]. The purity of these recombinant proteins was examined by using 12% SDS-PAGE followed by Coomassie staining (C). Molecular weight (MW) is shown in kDa.