Malaria Journal

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Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods

Chris Bass1*, Dimitra Nikou2, Martin J Donnelly2, Martin S Williamson1, Hilary Ranson3, Amanda Ball2, John Vontas4 and Linda M Field1

Author Affiliations

1 Department of Biological Chemistry, Rothamsted Research, Harpenden, AL5 2JQ, UK

2 Vector Group, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L35QA, UK

3 Division of Cell and Molecular Biology, Sir Alexander Flemming Building, Imperial College, London, UK

4 Laboratory of Pesticide Science, Agricultural University of Athens, Iera Odos 75, 118 55, Votanikos, Athens, Greece

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Malaria Journal 2007, 6:111 doi:10.1186/1475-2875-6-111

Published: 13 August 2007

Additional files

Additional file 1:

Excel spreadsheet showing the details of the 96 sample reference plate and full results of the blind genotyping trial. The genotype assigned to each sample by each assay is shown. S: Wild type allele (L1014), Rw: Resistant allele, West African mutation (L1014F), Re: Resistant allele, East African mutation (L1014S). Negative controls (water or Plasmodium falciparum DNA) are highlighted in grey. Failed reactions are highlighted in blue. Incorrectly scored reactions are highlighted in yellow.

Format: XLS Size: 49KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data