Figure 1.

Fluorescent intensity (Rn) of the FAM reporter dye (blue line) compared to VIC reporter dye (green line) as PCR amplification cycles proceed (x axes). Fluorescent intensity is measured as Rn (y-axes), the absolute fluorescence of the reporter dye divided by that of the passive reference dye, Rox. An increase in FAM indicates a wild type population [86(e), 184(f), 1034(g), 1042(h)] while an increase in VIC indicates a mutant population [86(a), 184(b), 1034(c), 1042(d)]. Polyclonal infections may be identified by an increase in fluorescent intensity of both reporter dyes (not shown). Mutant 1034 DNA (c) was obtained from a previous study [6] because none of the samples in this study contained that mutation.