Email updates

Keep up to date with the latest news and content from Malaria Journal and BioMed Central.

Open Access Research

An analysis of timing and frequency of malaria infection during pregnancy in relation to the risk of low birth weight, anaemia and perinatal mortality in Burkina Faso

Innocent Valea1*, Halidou Tinto12, Maxime K Drabo2, Lieven Huybregts3, Hermann Sorgho2, Jean-Bosco Ouedraogo12, Robert T Guiguemde1, Jean Pierre van Geertruyden4, Patrick Kolsteren35, Umberto D'Alessandro6 and the FSP/MISAME study Group

Author Affiliations

1 Laboratory of Parasitology and Entomology, Centre Muraz, Bobo-Dioulasso, Burkina Faso

2 Institut de Recherche en Sciences de la Santé, Direction Régionale de l'Ouest, Bobo-Dioulasso, Burkina Faso

3 Department of Food Safety and Food Quality, Ghent University, Ghent, Belgium

4 Department of International Health, University of Antwerp, Antwerp, Belgium

5 Unit of Nutrition and Child Health, Department of Public Health, Prince Leopold Institute of Tropical Medicine, Antwerp, Belgium

6 Unit of Epidemiology and Control of Parasitic Diseases, Department of Parasitology, Prince Leopold Institute of Tropical Medicine, Antwerp, Belgium

For all author emails, please log on.

Malaria Journal 2012, 11:71  doi:10.1186/1475-2875-11-71


The electronic version of this article is the complete one and can be found online at: http://www.malariajournal.com/content/11/1/71


Received:13 November 2011
Accepted:16 March 2012
Published:16 March 2012

© 2012 Valea et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

A prospective study aiming at assessing the effect of adding a third dose sulphadoxine-pyrimethamine (SP) to the standard two-dose intermittent preventive treatment for pregnant women was carried out in Hounde, Burkina Faso, between March 2006 and July 2008. Pregnant women were identified as earlier as possible during pregnancy through a network of home visitors, referred to the health facilities for inclusion and followed up until delivery.

Methods

Study participants were enrolled at antenatal care (ANC) visits and randomized to receive either two or three doses of SP at the appropriate time. Women were visited daily and a blood slide was collected when there was fever (body temperature > 37.5°C) or history of fever. Women were encouraged to attend ANC and deliver in the health centre, where the new-born was examined and weighed. The timing and frequency of malaria infection was analysed in relation to the risk of low birth weight, maternal anaemia and perinatal mortality.

Results

Data on birth weight and haemoglobin were available for 1,034 women. The incidence of malaria infections was significantly lower in women having received three instead of two doses of SP. Occurrence of first malaria infection during the first or second trimester was associated with a higher risk of low birth weight: incidence rate ratios of 3.56 (p < 0.001) and 1.72 (p = 0.034), respectively. After adjusting for possible confounding factors, the risk remained significantly higher for the infection in the first trimester of pregnancy (adjusted incidence rate ratio = 2.07, p = 0.002). The risk of maternal anaemia and perinatal mortality was not associated with the timing of first malaria infection.

Conclusion

Malaria infection during first trimester of pregnancy is associated to a higher risk of low birth weight. Women should be encouraged to use long-lasting insecticidal nets before and throughout their pregnancy.

Keywords:
Malaria infection; Pregnancy; First trimester; Sulphadoxine-pyrimethamine; IPT

Background

Each year, about 30 million pregnant women are at risk for malaria [1], with consequences of public health concern [2]. Indeed, malaria infection may result in maternal anaemia [3], pre-term delivery and low birth weight (LBW) [2,4] and is therefore an important determinant of perinatal mortality [5-7]. To prevent and manage malaria in pregnancy, the Word Health Organization (WHO) recommends effective case management, use of insecticide-treated bed nets and intermittent preventive treatment (IPTp) with sulphadoxine-pyrimethamine (SP) [8]. Following the latest WHO guidelines, IPTp should be administered at least twice, during the second and third trimester. In Burkina Faso in 2005, the Ministry of Health (MoH) replaced weekly chloroquine chemoprophylaxis, whose efficacy had declined because of widespread resistance [9], with IPTp/SP [10]. Several studies have demonstrated the excellent safety and efficacy of IPTp [11-15]. Most studies used maternal anaemia and LBW as impact indicators. IPTp prevents the adverse effects of malaria during pregnancy by clearing any active infection and providing post-treatment prophylaxis. The length of protection may become shorter and infection may occur progressively earlier as SP resistance increases [16]. As SP is given at least twice during pregnancy, its prophylactic efficacy may vary according to both the timing of malaria infection and SP administration. Unfortunately, because of fears of possible toxicity, SP cannot be given during the first trimester of pregnancy. However, there is also little information on the consequence of malaria infection during the first trimester as most information available refers to infections occurring in the second and third trimester [17-20]. A randomized trial comparing two doses versus three doses of IPTp/SP was carried out in rural Burkina Faso. Participants were recruited as early as possible during pregnancy and followed-up prospectively until delivery, allowing an estimation of the effect of malaria infection at different gestational ages on the risk of maternal anaemia, LBW and perinatal mortality. Results are reported below.

Methods

Study settings

Two peripheral health centres (Koho and Karaba) in the Houndé health district, south-west Burkina-Faso, were selected for the study. In this area, malaria is markedly seasonal with high transmission during the rainy season (June to December) [21]. The district hospital and the 28 peripheral health facilities cover a population of approximately 247,500 people. In 2007, the number of pregnant women at risk of malaria was estimated at 12,500, while malaria was the main disease in the health district, accounting for about 38% of all consultations and 52% of hospitalizations.

Study design

This was part of a larger study investigating both the effect of multiple micronutrients supplementation (MMS) versus fortified food supplementation (FFS) and that of IPTp/SP, two versus three doses on the health of pregnant women and that of their offspring. Participants were randomized in permuted blocks of four to receive either two doses of SP as recommended by the National Malaria Control Programme (NMCP) or three doses. Randomization numbers were generated by a computer program, sealed in opaque envelopes and opened only when an eligible subject was identified. These numbers were then transmitted to the study pharmacist who packaged the drugs in individual plastic zip bags. Each bag was labelled with the participant's name, identification, residence, and randomization group. The field pharmacist prepared for each woman an individual schedule for SP administration according to the gestational age at randomization and transmitted it to the trained home visitors who administered both SP and either MMS or FSS. Results of the nutrition intervention have been reported elsewhere [22]. The effect of IPTp with three doses of SP versus two doses of SP on LBW and pregnancy outcomes have also been reported in a previous publication [23].

Study participants

Pregnant women were identified through a community-based network of home visitors, as described elsewhere [22]. All women of child-bearing age in the study area were identified and paid monthly visits during which they were screened for pregnancy. Women suspected to be pregnant were referred to the health facilities for a pregnancy test. The study protocol and procedures were then explained to the potential participants in the local language and those agreeing to participate were asked to provide a written inform consent. Women with known hypersensibility to SP or not planning to stay in the study area for the next two years were excluded.

Recruitment and follow-up

Study participants were recruited at ANC clinics, where the health staff recorded demographic data, medical and pregnancy history. In addition, a clinical examination was performed and vital signs, weight, height and arm circumference were measured. All measures were repeated at each ANC visit. Gestational age was assessed as early as possible by the study obstetrician using trans-abdominal ultrasound fetal biometry. Women included in the study were visited at home daily by the home visitors who recorded the body temperature and registered any complaint. In case of fever (body temperature ≥ 37.5°C) or history of fever since the last visit, a blood sample for thick and thin film was collected and sent to the health district laboratory. All women with a confirmed malaria infection were treated with a full course of quinine (24 mg/kg/day for seven days), regardless of their gestational age. Pregnant women were encouraged to attend their scheduled ANC visits and to deliver at the health facilities where the new-borns was examined, weighed and measured twice by two different members of the health staff.

Laboratory methods

Thick and thin blood smears were collected in duplicate and stained with Giemsa 10% (pH 7.2) for 10 minutes. Parasite densities were determined on the thick smears by counting asexual parasites per 200 white blood cells (WBC) and assuming a WBC count of 8,000/μl. A thick blood smear was considered negative after reading 100 high-power fields. Ten percent of the slides were randomly selected and sent to the Laboratory of Parasitology/Centre Muraz for quality control. Haemoglobin (Hb) was measured by using a portable spectrophotometer (HemoCue, Angelholm, Sweden).

Ethics

The study protocol was approved by the ethical committees of the Centre Muraz, Bobo-Dioulasso, Burkina Faso, and the University of Antwerp, Belgium. The trial was registered in the ClinicalTrial.gov registry (identifier: NCT00909974). The study purpose and procedures where explained to the potential participants by the study clinician in the local languages. Women who fulfilled the inclusion criteria and agreed to participate in the study were asked to provide a written inform consent.

Definitions and statistical analysis

A new-born was classified as LBW if the birth weight was < 2,500 g. Women with haemoglobin levels < 11 g/dl were classified as anaemic, those with haemoglobin levels < 8 g/dl were classified as having moderate-to-severe anaemia. Neonatal death was defined as one occurring between delivery and 28 days of life. Malaria infection was defined as a slide positive for Plasmodium falciparum, any density. Incidence rates were computed considering the follow-up duration time (in months) of each woman. Women with a malaria infection after previous treatment with quinine were considered as re-infected. Time to re-infection was defined as the period between the first infection parasite clearance and the second infection. As the parasite clearance was not monitored, we calculated an adjusted time to re-infection considering that all parasites were cleared after three days as indicated in a previous publication [24].

Data were double entered in a Microsoft Access® database by two data clerks. Validation and analysis were performed using Stata 10 IC® software. Only singleton pregnancies were included in the analysis. The Chi2 or Fisher exact test were used to compare proportions for categorical variables while a t-test for normally distributed or Mann-Whitney test for non-normally distributed was used for continuous variables at baseline. A multivariate analysis using a Poisson regression model with robust standard error estimates to evaluate the relationship between explanatory variables and outcomes was also performed. Crude and adjusted incidence rate ratios (IRR) with 95% CI were computed. A p-value ≤ 0.05 was considered as statistically significant.

The timing of malaria infection was defined according to the first infection detected at the first, second or third trimester of pregnancy. The frequency of malaria infection was categorized according to the number of positive slides detected during the whole pregnancy, i.e. 0, 1, or ≥ 2. Explanatory variables and possible confounders included the number of SP doses received (two or three), the nutritional supplementation (FFS or MMS), gravidity, body mass index at enrolment (underweight: body mass index < 18.5 kg/m2; or normal weigh, BMI ≥ 18.5 kg/m2), and malaria transmission season at delivery (low transmission season between December and May and high transmission season between June and November).

Results

Study population

From March 2006 to July 2008, 1,296 women were randomized into the study. Table 1 shows the baseline characteristics of study population according to the timing of first infection. Characteristics of study participants according to randomization groups were published elsewhere [23]. A total of 1,053 women attended at least three ANC visits while 121 and 100 attended two and one ANC visit, respectively. Analysis was performed on 1,034 women with complete records on birth weight and haemoglobin. The mean maternal age was 24.4 years and the mean gestational age at enrolment was 15.9 weeks. There was no difference in terms of baselines characteristics between women included in the analysis and those excluded for missing data.

Table 1. Characteristic of study participants at inclusion by timing of first infection

Incidence rates of malaria detected infections

The overall incidence rate (IR) of malaria infection (expressed per 1,000 women-months) was 39.2, and was significantly higher in primi- (88.6, 95%CI: 72.6-108.1) than in secundi- (50.6, 95%CI: 39.1-65.5) and multi-gravidae (18.8, 95%CI: 14.7-24.1) (Table 2). The IR of malaria infection was significantly lower (p = 0.014) in women having received three (23.8%, 95%CI: 15.5-36.4) than two SP doses (46.6%, 95%CI: 38.3-55.4). No difference between the MMS group (40.4, 95%CI: 33.5-48.6) and the FFS group (38.0%, 95%CI: 31.5-45.9) was found (p = 0.42).

Table 2. Number of cases and incidence rates of malaria infections in the study population (per 1000 women-month)

Rates of re-infection and length of time-to-reinfection

The overall incidence of re-infections after quinine treatment was 11.4% (95%CI: 8.9-14.6) and was significantly higher (p < 0.001) in primi- (34.7, 95%CI: 25.3-47.7) than in secundi- (11.3, 95%CI: 6.6-19.5) and multi-gravidae (3.6%, 95%CI: 2.1-6.4). Re-infection tended to be lower in women having received three (6.8%, 95%CI: 3.1-15.1) than two (14.3%, 95%CI: 10.2-19.9) SP doses (p = 0.06). No difference between MMS and FFS group was found (data not shown).

Overall, the adjusted mean time to re-infection was 59.3 days (95%CI: 45.8-72.7) with no difference by gravidity, i.e. primi- 63.7 days (95%CI: 47.2-80.2), secundi- 40.7 days (95%CI: 07.9-73.4), and multi-gravidae 51.2 days (95%CI: 3.2-99.1). Mean time to reinfection was not analysed by number of SP doses as only four women in the three-dose group were re-infected.

Effect of timing and frequency of malaria infection on low birth weight, maternal anaemia and perinatal mortality

When considering the timing of the first infection, no significant difference could be found for maternal anemia and perinatal mortality, while women infected both in the first (IRR = 3.56, p < 0.001) and the second trimester (IRR = 1.72, p = 0.034) had a significantly higher risk of delivering a LBW baby as compared to women without any infection (Table 3). After adjusting for age, parity, body mass index, number of SP doses received and malaria season at delivery, such risk remained significantly higher for women infected in the first trimester of pregnancy (adjusted IRR = 2.07, p = 0.002) (Table 3). Also, women infected in first trimester had a significantly higher risk of delivering a LBW when compared to women infected in the third trimester (Crude IRR = 3.09, 95%CI: 1.66-5.73, p < 0.01; and adjusted IRR = 3.27, 95%CI: 1.78-6.01, p < 0.01) while no difference was observed between women infected in the second trimester and women infected in the third trimester (Crude IRR = 1.49, 95%CI: 0.78-2.87, p = 0.23 and Adjusted IRR = 1.58, 95%CI: 0.83-2.98; p = 0.16).

Table 3. Crude and adjusted incidence rate ratios (IRR) of low birth weight, maternal anaemia and perinatal mortality by trimester of malaria infection using Poisson regression

Most women had just one infection though some of them experienced up to four infections until delivery. Though the LBW risk seemed to be significantly higher in women with two or more infections as shown by the crude IRR, the difference disappeared after adjusting for women age, parity, body mass index, number of SP doses received and malaria season at delivery (Table 4). Similarly, the risk for maternal anaemia, though apparently increased by two or more infection, did not vary significantly with the number of re-infections after adjustment by the variables mentioned above. The risk of perinatal mortality was similar for women with ≥ 1 infection and those with no infection (adjusted IRR = 0.87, 95% CI 0.27-2.83) (Table 4).

Table 4. Crude and adjusted incident rate ratios (IRR) of low birth weight, maternal anaemia and perinatal mortality by number of detected malaria infection using Poisson regression

Discussion

The risk of delivering a LBW baby was significantly higher in women infected during the first trimester of pregnancy, even after adjusting for several potential confounding variables such as parity and number of IPTp/SP doses received. Thought the pathogenesis and immunity of malaria in pregnancy has been explored, the contribution of placental immuno-pathology to anaemia and LBW is not fully understood [25]. Plasmodium falciparum-infected erythrocytes sequester in the placenta through adhesion mechanisms, inducing placental inflammatory responses, particularly monocytes infiltrates. Inflammatory cytokines produced by T cell and macrophages, in particular Th1 responses are associated with maternal anaemia, spontaneous abortions and premature deliveries. These cytokines are known to help eliminate the parasites from the placenta but their overproduction can threaten the pregnancy [25,26]. Acute infection, particularly with high parasites densities have been associated with preterm delivery while chronic infection have been associated with LBW due to intrauterine growth retardation (IUGR) and severe anaemia [27]. Decreased placental growth and/or decreased nutrient transport have been suggested as the possible final common pathways by which malaria leads to IUGR [25,28]. In this study, malaria infection during the first trimester was strongly associated with LBW. It is unclear whether this is due to a specific consequence of the infection at this particular time, or whether it is related to the higher risk these women have throughout their pregnancy. In any case, the currently recommended IPT/SP does not cover the first trimester of pregnancy as the first SP dose should not be given before quickening, at about 20 weeks of gestation, for fear of possible toxicity for the foetus [29]. There is little information on the use of SP during the first trimester of pregnancy; available reports are limited to small case series from developed countries [15]. Therefore, the only option available for protecting pregnant women during this vulnerable period is the use of long-lasting insecticidal nets (LLIN) whose coverage and use in sub-Saharan Africa, despite major efforts such as campaigns of free mass distribution, is less than optimal [30,31]. A potentially interesting additional strategy might be the systematic screening and treatment of all pregnant women and this option was recently investigated [32,33]. However, when considering the difficulty of identifying women at the earliest stage of their pregnancy [34], such an approach would hardly solve the problem of protecting during the first trimester. Therefore, promoting LLIN through community-based promotional campaigns targeted not only at pregnant women but also at adolescents seems to be the only option available to tackle this specific problem.

The rate of re-infection and the time length to re-infection were suggested as possible indicators to evaluate the preventive efficacy of SP [35]. In this study, the incidence of re-infections was much higher in women having received two as compared to three doses of SP, indicating that the latter is probably more efficacious. Nevertheless, the rate of re-infection was established after treatment with quinine and does not necessarily represent the true preventive efficacy of SP.

The study has some limitations. Information on LLIN ownership and use was not collected and, knowing that this has an impact on the malaria risk during pregnancy [36], it should probably have been considered as an important confounding factor. However, it is unlikely that this has resulted in biased estimates as LLIN use in the study population was probably very low at the time of the study. In Boromo, a neighbouring district, LLIN use was estimated at about 27% in the high transmission season [21].

Malaria infection during pregnancy was probably underestimated as it was detected by peripheral blood smears. Placental biopsies were collected during the study but could not be analyzed due to budget constraints.

Despite the network of home visitors implemented and the daily visits carried out for an earlier identification of pregnant women, most of those detected during the first trimester were at ≥ 10 weeks of gestation, a limiting factor for the assessment of infections during the first trimester. For some reasons, mainly socio-cultural factors [34], women were reluctant to declare their pregnancy earlier and this should be further investigated.

In conclusion, malaria infections occurring in the first trimester of pregnancy seem to have an important effect on LBW, though the mechanisms are not yet understood. Women should be encouraged to use LLIN throughout their pregnancy so that the deleterious effect of malaria infection during the first trimester could be prevented.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

IV participated in study coordination and data cleaning, performed the data analysis and drafted the manuscript. HT participated in the design, study coordination and corrected the manuscript. MKD, HS and JPG corrected the manuscript. LH participated in data cleaning and corrected the manuscript. RTG and KP participated in the design of the study. UDA participated in the design, helped in data analysis and corrected the manuscript. All authors read and approved the final manuscript.

Acknowledgements

The FSP/MISAME Study Group thanks the community and especially the women from Karaba and Koho and the families who participated in the study, the health staff of the Houndé district, and the staff of Center Muraz for their great support.

The FSP/MISAME Study Group also acknowledges the study funding organizations (Nutrition Third Word, The Belgium ministry of development, Flemish Interuniversity Council, and French Ministry of Development) for their financial support to the study.

We acknowledge the FSP/MISAME field investigator team (J-P Ki and V Koudougbo/Valea, sociologists; L Toe and H Lanou, physicians; E Da, obstetrician-gynecologist; G Lougue, pharmacist; B Nagalo and O Guebe, nurses; and B Hien, laboratory technician), and the logistic team (S Ouattara, study coordinator; J Bicaba, administrative assistant; C Kouakou Yameogo; N Diallo, data clerk; M Sanou, driver; and A Hien, pharmacist).

References

  1. Dellicour S, Tatem AJ, Guerra CA, Snow RW, ter Kuile FO: Quantifying the number of pregnancies at risk of malaria in 2007: a demographic study.

    PLoS Med 2010, 7:e1000221. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  2. Desai M, ter Kuile FO, Nosten F, McGready R, Asamoa K, Brabin B, Newman RD: Epidemiology and burden of malaria in pregnancy.

    Lancet Infect Dis 2007, 7:93-104. PubMed Abstract | Publisher Full Text OpenURL

  3. Menendez C, Fleming AF, Alonso PL: Malaria-related anaemia.

    Parasitol Today 2000, 16:469-476. PubMed Abstract | Publisher Full Text OpenURL

  4. Guyatt HL, Snow RW: Impact of malaria during pregnancy on low birth weight in sub-Saharan Africa.

    Clin Microbiol Rev 2004, 17:760-9.

    table

    PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  5. van Geertruyden JP, Thomas F, Erhart A, d'Alessandro U: The contribution of malaria in pregnancy to perinatal mortality.

    Am J Trop Med Hyg 2004, 71:35-40. PubMed Abstract | Publisher Full Text OpenURL

  6. McCormick MC: The contribution of low birth weight to infant mortality and childhood morbidity.

    N Engl J Med 1985, 312:82-90. PubMed Abstract | Publisher Full Text OpenURL

  7. Uneke CJ: Impact of placental Plasmodium falciparum malaria on pregnancy and perinatal outcome in sub-Saharan Africa: I: introduction to placental malaria.

    Yale J Biol Med 2007, 80:39-50. PubMed Abstract | PubMed Central Full Text OpenURL

  8. WHO/AFRO: A strategic framework for malaria prevention and control during pregnancy in the African Region. Word Health Organisation Regional Office for Africa. Brazzaville; 2004. OpenURL

  9. Sirima SB, Sawadogo R, Moran AC, Konate A, Diarra A, Yameogo M, Parise ME, Newman RD: Failure of a chloroquine chemoprophylaxis program to adequately prevent malaria during pregnancy in Koupela District, Burkina Faso.

    Clin Infect Dis 2003, 36:1374-1382. PubMed Abstract | Publisher Full Text OpenURL

  10. Ministere de la Santé/Programme National de Lutte contre le Paludisme:

    Directives Nationales pour la prise en charge du paludisme au Burkina Faso. 2006. OpenURL

  11. Gies S, Coulibaly SO, Ouattara FT, d'Alessandro U: Individual efficacy of intermittent preventive treatment with sulfadoxine-pyrimethamine in primi- and secundigravidae in rural Burkina Faso: impact on parasitaemia, anaemia and birth weight.

    Trop Med Int Health 2009, 14:174-182. PubMed Abstract | Publisher Full Text OpenURL

  12. Shulman CE, Dorman EK, Cutts F, Kawuondo K, Bulmer JN, Peshu N, Marsh K: Intermittent sulphadoxine-pyrimethamine to prevent severe anaemia secondary to malaria in pregnancy: a randomised placebo-controlled trial.

    Lancet 1999, 353:632-636. PubMed Abstract | Publisher Full Text OpenURL

  13. Sirima SB, Cotte AH, Konate A, Moran AC, Asamoa K, Bougouma EC, Diarra A, Ouedraogo A, Parise ME, Newman RD: Malaria prevention during pregnancy: assessing the disease burden one year after implementing a program of intermittent preventive treatment in Koupela District, Burkina Faso.

    Am J Trop Med Hyg 2006, 75:205-211. PubMed Abstract | Publisher Full Text OpenURL

  14. Tiono AB, Ouedraogo A, Bougouma EC, Diarra A, Konate AT, Nebie I, Sirima SB: Placental malaria and low birth weight in pregnant women living in a rural area of Burkina Faso following the use of three preventive treatment regimens.

    Malar J 2009, 8:224. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  15. Peters PJ, Thigpen MC, Parise ME, Newman RD: Safety and toxicity of sulfadoxine/pyrimethamine: implications for malaria prevention in pregnancy using intermittent preventive treatment.

    Drug Saf 2007, 30:481-501. PubMed Abstract | Publisher Full Text OpenURL

  16. White NJ: How antimalarial drug resistance affects post-treatment prophylaxis.

    Malar J 2008, 7:9. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  17. Kalilani L, Mofolo I, Chaponda M, Rogerson SJ, Meshnick SR: The effect of timing and frequency of Plasmodium falciparum infection during pregnancy on the risk of low birth weight and maternal anemia.

    Trans R Soc Trop Med Hyg 2010, 104:416-422. PubMed Abstract | Publisher Full Text OpenURL

  18. Cottrell G, Mary JY, Barro D, Cot M: The importance of the period of malarial infection during pregnancy on birth weight in tropical Africa.

    Am J Trop Med Hyg 2007, 76:849-854. PubMed Abstract | Publisher Full Text OpenURL

  19. Cot M, Abel L, Roisin A, Barro D, Yada A, Carnevale P, Feingold J: Risk factors of malaria infection during pregnancy in Burkina Faso: suggestion of a genetic influence.

    Am J Trop Med Hyg 1993, 48:358-364. PubMed Abstract | Publisher Full Text OpenURL

  20. Huynh BT, Fievet N, Gbaguidi G, Dechavanne S, Borgella S, Guezo-Mevo B, Massougbodji A, Ndam NT, Deloron P, Cot M: Influence of the timing of malaria infection during pregnancy on birth weight and on maternal anemia in Benin.

    Am J Trop Med Hyg 2011, 85:214-220. PubMed Abstract | Publisher Full Text OpenURL

  21. Coulibaly SO, Gies S, d'Alessandro U: Malaria burden among pregnant women living in the rural district of Boromo, Burkina Faso.

    Am J Trop Med Hyg 2007, 77:56-60. PubMed Abstract | Publisher Full Text OpenURL

  22. Huybregts L, Roberfroid D, Lanou H, Menten J, Meda N, Van CJ, Kolsteren P: Prenatal food supplementation fortified with multiple micronutrients increases birth length: a randomized controlled trial in rural Burkina Faso.

    Am J Clin Nutr 2009, 90:1593-1600. PubMed Abstract | Publisher Full Text OpenURL

  23. Valea I, Tinto H, Drabo MK, Huybregts L, Henry MC, Roberfroid D, Guiguemde RT, Kolsteren P, d'Alessandro U: Intermittent preventive treatment of malaria with sulphadoxine-pyrimethamine during pregnancy in Burkina Faso: effect of adding a third dose to the standard two-dose regimen on low birth weight, anaemia and pregnancy outcomes.

    Malaria Journal 2010, 9:324. OpenURL

  24. Piola P, Nabasumba C, Turyakira E, Dhorda M, Lindegardh N, Nyehangane D, Snounou G, Ashley EA, McGready R, Nosten F, Guerin PJ: Efficacy and safety of artemether-lumefantrine compared with quinine in pregnant women with uncomplicated Plasmodium falciparum malaria: an open-label, randomised, non-inferiority trial.

    Lancet Infect Dis 2010, 10:762-769. PubMed Abstract | Publisher Full Text OpenURL

  25. Rogerson SJ, Hviid L, Duffy PE, Leke RF, Taylor DW: Malaria in pregnancy: pathogenesis and immunity.

    Lancet Infect Dis 2007, 7:105-117. PubMed Abstract | Publisher Full Text OpenURL

  26. Wegmann TG, Lin H, Guilbert L, Mosmann TR: Bidirectional cytokine interactions in the maternal-fetal relationship: is successful pregnancy a TH2 phenomenon?

    Immunol Today 1993, 14:353-356. PubMed Abstract | Publisher Full Text OpenURL

  27. Brabin BJ, Romagosa C, Abdelgalil S, Menendez C, Verhoeff FH, McGready R, Fletcher KA, Owens S, d'Alessandro U, Nosten F, Fisher PR, Ordi J: The sick placenta-the role of malaria.

    Placenta 2004, 25:359-378. PubMed Abstract | Publisher Full Text OpenURL

  28. Rogerson SJ, Mwapasa V, Meshnick SR: Malaria in pregnancy: linking immunity and pathogenesis to prevention.

    Am J Trop Med Hyg 2007, 77:14-22. PubMed Abstract | Publisher Full Text OpenURL

  29. Briand V, Cottrell G, Massougbodji A, Cot M: Intermittent preventive treatment for the prevention of malaria during pregnancy in high transmission areas.

    Malar J 2007, 6:160. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  30. Bourgoing R: Bednets for malaria. Reports from the field -- Africa.

    Glob Impacts 1997, 14-15. OpenURL

  31. Noor AM, Mutheu JJ, Tatem AJ, Hay SI, Snow RW: Insecticide-treated net coverage in Africa: mapping progress in 2000-07.

    Lancet 2009, 373:58-67. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  32. Tagbor H, Bruce J, Agbo M, Greenwood B, Chandramohan D: Intermittent screening and treatment versus intermittent preventive treatment of malaria in pregnancy: a randomised controlled non-inferiority trial.

    Plos One 2010, 5:e14425. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  33. Smith LA, Jones C, Adjei RO, Antwi GD, Afrah NA, Greenwood B, Chandramohan D, Tagbor H, Webster J: Intermittent screening and treatment versus intermittent preventive treatment of malaria in pregnancy: user acceptability.

    Malar J 2010, 9:18. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  34. Grietens KP, Gies S, Coulibaly SO, Ky C, Somda J, Toomer E, Muela RJ, d'Alessandro U: Bottlenecks for high coverage of intermittent preventive treatment in pregnancy: the case of adolescent pregnancies in rural Burkina Faso.

    Plos One 2010, 5:e12013. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  35. Brabin BJ, Warsame M, Uddenfeldt-Wort U, Dellicour S, Hill J, Gies S: Monitoring and evaluation of malaria in pregnancy-developing a rational basis for control.

    Malar J 2008, 7(Suppl 1):S6. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  36. d'Alessandro U: Insecticide treated bed nets to prevent malaria.

    BMJ 2001, 322:249-250. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL