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An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum
Sandra Hasenkamp, Eleanor H Wong, Paul Horrocks Malaria Journal 2012, 11:42 (10 February 2012)
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Editor’s summary
Optimized method to do luciferase assays with P. falciparum blood stage cultures. The presented method is simpler and removes a lot of the variation observed with the standard assay.
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Using CF11 cellulose columns to inexpensively and effectively remove human DNA from Plasmodium falciparum-infected whole blood samples
Meera Venkatesan, Chanaki Amaratunga, Susana Campino, Sarah Auburn, Oliver Koch, Pharath Lim, Sambunny Uk, Duong Socheat, Dominic P Kwiatkowski, Rick M Fairhurst, Christopher V Plowe Malaria Journal 2012, 11:41 (10 February 2012)
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Editor’s summary
Ddescribes a methodological advance in the purification of Plasmodium falciparum DNA and depletion of accompanying human DNA, to generate material sufficient for next-generation sequencing platforms
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Malaria transmission in Libreville: results of a one year survey
Jean Romain Mourou, Thierry Coffinet, Fanny Jarjaval, Christelle Cotteaux, Eve Pradines, Godefroy Lydie, Maryvonne Kombila, Frederic Pages Malaria Journal 2012, 11:40 (9 February 2012)
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Editor’s summary
Clinical studies have shown a decline in the burden of malaria in Libreville, the capital city of Gabon, but the characteristics of transmission are poorly known. It appears that the decrease of transmission seen inperi-urban areas is probably more dependent of the socio-economic level of a quarter than of its location in the city.
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Combination of probenecid-sulphadoxine-pyrimethamine for intermittent preventive treatment in pregnancy
Julie Gutman, S Patrick Kachur, Laurence Slutsker, Alexis Nzila, Theonest Mutabingwa Malaria Journal 2012, 11:39 (9 February 2012)
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Editor’s summary
This paper discusses the potential role of adding probenecid in improving SP efficacy in IPTp, thus potentially extending the lifespan of SP in its applications as the choice drug for for intermittent preventive treatment.
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High IFN-gamma and TNF production by peripheral NK cells of Colombian patients with different clinical presentation of Plasmodium falciparum
Olga Agudelo, Julio Bueno, Andres Villa, Amanda Maestre Malaria Journal 2012, 11:38 (8 February 2012)
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Editor’s summary
The paper adds to our knowledge of field-based, clinically oriented, NK cell subsets and cytokine production related to malarial innate immunity.
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The magnitude and trend of artemether-lumefantrine stock-outs at public health facilities in Kenya
Raymond K Sudoi, Sophie Githinji, Andrew Nyandigisi, Alex Muturi, Robert W Snow, Dejan Zurovac Malaria Journal 2012, 11:37 (8 February 2012)
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A paper which helps to understand how supply is organized from central to peripheral level, and how this might affect the supply of peripheral units.
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Human Plasmodium knowlesi infection in Ranong province, southwestern border of Thailand
Natthawan Sermwittayawong, Balbir Singh, Mitsuaki Nishibuchi, Nongyao Sawangjaroen, Varaporn Vuddhakul Malaria Journal 2012, 11:36 (8 February 2012)
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Editor’s summary
Plasmodium knowlesi infection in humans along the southwestern border of Thailand, including interesting discussion on the possible changes in epidemiology.
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Human cerebral malaria and Plasmodium falciparum genotypes in Malawi
Danny A Milner, Jimmy Vareta, Clarissa Valim, Jacqui Montgomery, Rachel F Daniels, Sarah K Volkman, Daniel E Neafsey, Daniel J Park, Stephen F Schaffner, Nira C Mahesh, Kayla G Barnes, David M Rosen, Amanda K Lukens, Daria Van Tyne, Roger C Wiegand, Pardis C Sabeti, Karl B Seydel, Simon J Glover, Steve Kamiza, Malcolm E Molyneux, Terrie E Taylor, Dyann F Wirth Malaria Journal 2012, 11:35 (7 February 2012)
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Editor’s summary
An interesting study confirming that the parasite genetic complexity is lower in cerebral malaria than in patients with non-complicated malaria. In their study, the authors found that cerebral malaria patients harbour a parasite population dominated by a single variant identical throughout the blood and organs.
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Anti-malarial drugs: how effective are they against Plasmodium falciparum gametocytes?
Christopher L Peatey, Didier Leroy, Donald L Gardiner, Katharine R Trenholme Malaria Journal 2012, 11:34 (6 February 2012)
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Cytoadherence and virulence - the case of Plasmodium knowlesi malaria
Farrah A Fatih, Angela Siner, Atique Ahmed, Lu Chan Woon, Alister G Craig, Balbir Singh, Sanjeev Krishna, Janet Cox-Singh Malaria Journal 2012, 11:33 (3 February 2012)
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Editor’s summary
Another twist in the tale of cytoadherence ? Is in vitro cytoadherence really a marker of virulence, even in case of parasites, such as P. knowlesi or P. vivax, which are not normally sequestered ?
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Can universal insecticide-treated net campaigns achieve equity in coverage and use? The case of northern Nigeria
Yazoume Ye, Elizabeth Patton, Albert Kilian, Samantha Dovey, Erin Eckert Malaria Journal 2012, 11:32 (1 February 2012)
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Editor’s summary
Using data from a post-campaign survey, the authors investigated the effect of the mass distribution campaigns in achieving equity in household ownership and use ITNs
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Attractive toxic sugar bait (ATSB) methods decimate populations of Anopheles malaria vectors in arid environments regardless of the local availability of favoured sugar-source blossoms
John C Beier, Gunter C Muller, Weidong Gu, Kristopher L Arheart, Yosef Schlein Malaria Journal 2012, 11:31 (1 February 2012)
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Editor’s summary
This field study in the arid malaria-free oasis environment of Israel compares how the availability of a primary natural sugar source for mosquitoes - flowering Acacia trees - affects the efficacy of ATSB methods for mosquito control.
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Apoptosis of the fibrocytes type 1 in the spiral ligament and blood labyrinth barrier disturbance cause hearing impairment in murine cerebral malaria
Joachim Schmutzhard, Christian H Kositz, Rudolf Glueckert, Erich Schmutzhard, Annelies Schrott-Fischer, Peter Lackner Malaria Journal 2012, 11:30 (1 February 2012)
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Editor’s summary
The paper describes histopathological changes induced by a murine malaria infection and relates this with hearing loss. It uses a well-stablished experimental model and addresses a major issue for malaria patients.
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The complexities of malaria disease manifestations with a focus on asymptomatic malaria
Dolie D Laishram, Patrick L Sutton, Nutan Nanda, Vijay L Sharma, Ranbir C Sobti, Jane M Carlton, Hema Joshi Malaria Journal 2012, 11:29 (31 January 2012)
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Editor’s summary
The pathogenesis of malaria is complex, and the clinical presentation of disease ranges from severe malaria, to mild and uncomplicated, to asymptomatic infections. Asymptomatic malaria remains a major challenge for malaria control programmes.
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Strengthening the policy setting process for global malaria control and elimination
Bianca J D'Souza, Robert D Newman Malaria Journal 2012, 11:28 (27 January 2012)
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Editor’s summary
A new Thematic Series in Malaria Journal will, after each meeting of the Malaria Policy Advisory Committee (MPAC), a new WHO committee, publish its recommendations and the evidence to support them.
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Visual and olfactory associative learning in the malaria vector Anopheles gambiae sensu stricto
Nora Chilaka, Elisabeth Perkins, Frederic Tripet Malaria Journal 2012, 11:27 (27 January 2012)
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Editor’s summary
The paper investigated associative learning in Anopheles
gambiae in a laboratory bioassay using visual and olfactory cues. The results address an important knowledge gap in mosquito ecology and emphasize the role of associative memory for host finding and blood-feeding behaviour, with important potential implications for vector control.
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Hyponatraemia in imported malaria: the pathophysiological role of vasopressin
Ewout J Hoorn, Marlies E van Wolfswinkel, Dennis A Hesselink, Yolanda B de Rijke, Rob Koelewijn, Jaap J van Hellemond, Perry JJ van Genderen Malaria Journal 2012, 11:26 (26 January 2012)
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Editor’s summary
This is a retrospective study of 519 cases of imported malaria, examining patterns and mechanisms of associated hyponatraemia. The results, which builds on work by the same authors published last year, is timely, because the issue of fluid resuscitation of patients with malaria controversial.
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Comparing changes in haematologic parameters occurring in patients included in randomized controlled trials of artesunate-amodiaquine vs single and combination treatments of uncomplicated falciparum in sub-Saharan Africa
Julien Zwang, Jean-Louis Ndiaye, Abdoulaye Djimde, Grant Dorsey, Andreas Martensson, Corine Karema, Piero Olliaro Malaria Journal 2012, 11:25 (25 January 2012)
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Editor’s summary
Effect of different antimalarial treatments on haematologic parameters comparing artesunate-amodiaquine against various single treatment lines and combination therapies.
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Aging partially restores the efficacy of malaria vector control in insecticide-resistant populations of Anopheles gambiae s.l. from Burkina Faso
Christopher M Jones, Antoine Sanou, Wamdaogo M Guelbeogo, N'Fale Sagnon, Paul C D Johnson, Hilary Ranson Malaria Journal 2012, 11:24 (23 January 2012)
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Editor’s summary
The main finding here is that older mosquitoes are more susceptible to insecticides. This has important implications for understanding the epidemiological impact of resistance in field-based vector control interventions.
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Development of a TaqMan Allelic Discrimination Assay for detection of Single Nucleotides Polymorphisms associated with anti-malarial drug resistance
Edwin Kamau, Saba Alemayehu, Karla C Feghali, LaDonna S Tolbert, Bernard Ogutu, Christian F Ockenhouse Malaria Journal 2012, 11:23 (20 January 2012)
Abstract | Full text | PDF | PubMed
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Editor’s summary
Paper reporting the development of a TaqMan Real Time PCR assay for the detection of SNPs associated with anti-malarial resistance. This assay has promise as a convenient and efficient way to examine patient samples in resource-constrained settings.
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Plate-based transfection and culturing technique for genetic manipulation of Plasmodium falciparum
Florence Caro, Mathew G. Miller, Joseph L. DeRisi Malaria Journal 2012, 11:22 (18 January 2012)
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Editor’s summary
Paper describing a series of semi-automated protocols that will facilitate transfection of P. falciparum.
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Integrated vector management for malaria control in Uganda: knowledge, perceptions and policy development
Clifford M Mutero, Dieter Schlodder, Narcis Kabatereine, Randall Kramer Malaria Journal 2012, 11:21 (14 January 2012)
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Editor’s summary
This paper focuses on status of Integrated Vector Management in Uganda and is a good demonstration how surveys of individuals in different sectors can advance the development of IVM-based programmes in African countries.
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Importance of factors determining the effective lifetime of a mass, long-lasting, insecticidal net distribution: a sensitivity analysis
Olivier JT Briƫt, Diggory Hardy, Thomas A Smith Malaria Journal 2012, 11:20 (13 January 2012)
Abstract | Full text | PDF | PubMed
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Editor’s summary
Use of a pre-existing agent-based mathematical modelling platform for malaria to extend knowledge that is based on field studies on net and insecticide decay to model and estimate the epidemiological lifetimes of insecticide-treated bed nets under field conditions. A topic about which rather little is actually known.
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Changes in malaria morbidity and mortality in Mpumalanga Province, South Africa (2001-2009): a retrospective study
Lindokuhle Ngomane, Christiaan de Jager Malaria Journal 2012, 11:19 (13 January 2012)
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Editor’s summary
This paper provides an interesting descriptive review of nine years (eight malaria seasons) of routine malaria data from Mpumalanga Province, South Africa.
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Distance to health services influences insecticide-treated net possession and use among six to 59 month-old children in Malawi
Peter S Larson, Don P Mathanga, Carl H Campbell, Mark L Wilson Malaria Journal 2012, 11:18 (11 January 2012)
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Editor’s summary
The authors analysed the significance of distance and ITN possession/use through standard statistical tests. Strategies that exclusively distribute ITNs through health facilities are likely to be less effective in increasing possession and use in communities that are more distant from those facilities.
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