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1: Trans R Soc Trop Med Hyg. 1996 Nov-Dec;90(6):678-80.
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Direct evidence that asparagine at position 108 of the Plasmodium falciparum dihydrofolate reductase is involved in resistance to antifolate drugs in Tanzania.

Curtis J, Duraisingh MT, Trigg JK, Mbwana H, Warhurst DC, Curtis CF.

London School of Hygiene and Tropical Medicine, UK.

A nested polymerase chain reaction was used to amplify a fragment of the gene for dihydrofolate reductase of Plasmodium falciparum containing codon 108, where a point mutation, causing a serine to asparagine change, occurs in pyrimethamine resistant parasites. The presence of the mutation was detected by restriction enzyme digestion. Parasites in blood samples collected from asymptomatic children before, and 3 weeks after, treatment with pyrimethamine-sulfadoxine or chlorproguanil-dapsone were analysed. Parasites in the samples taken at 3 weeks carried only the asparagine mutant.

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PMID: 9015515 [PubMed - indexed for MEDLINE]