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HRP-2 deletion makes diagnostics further imperfect for low malaria transmission context in the Amazon Region (Jaime Chang, 01 April 2014)

Following on the 2010 report by Gamboa et al. on a large proportion of Plasmodium falciparum isolates from the Peruvian Amazon lacking HRP-2 and HRP-3 (DOI: 10.1371/journal.pone.0008091), colleagues from CDC and Amazon countries have assessed the frequency of deletion of HRP-2 and HRP-3 genes among P. falciparum strains circulating in Bolivia, Brazil, Colombia, Guyana, Peru, and Suriname. The deletions were found in parasites from all countries but Guyana. In the other countries HRP-2 deletion frequencies ranged between 4% and 33%, while HRP-3 deletion frequencies ranged between 3% and 53%. By adding a shortcoming to HRP-2 dependent rapid tests, this further complicates efforts against malaria in a region with decreasing malaria transmission, where most remaining malaria occurs in... read full comment

Comment on: Mosha et al. Malaria Journal, 12:221

Erratum: Error in the given primer concentrations. (Christel Gill Haanshuus, 08 March 2014)

We have unfortunately discover error in the given primer concentrations in our article. The incorrect primer concentrations affect the efficiency of the PCR... read full comment

Comment on: Haanshuus et al. Malaria Journal, 12:26

Vector species incrimination crucial for malaria elimination (Michael Bangs, 13 November 2013)

As regular readers of the Malaria Journal, we would like to point out an obvious weakness of the paper by Herdiana et al. (2013, 12:42) regarding the entomological analysis. Several aspects of the authors¿ conclusions are unacceptable, especially the conclusion that Anopheles dirus and An. minimus are present on Sabang Island (northern Sumatra). Even if true, such a pronouncement is inappropriate without performing definitive confirmation of species identity. Given they failed to do so, the findings should have been properly presented in the context of this shortcoming and what implications it might have for eliminating malaria from the island. Neither the subject paper nor cited reference (Hudson et al. 1985) indicates the primary malaria vector(s) on Sabang Island, other than to state... read full comment

Comment on: Herdiana et al. Malaria Journal, 12:42

Authors Note (Penelope Lynch, 19 March 2013)

A number of problems introduced during the copy-editing process for this paper remain unresolved in the currently published versions. We still hope to see these resolved by Biomed Central, but meanwhile note the following in an attempt to minimise confusion regarding the symbols representing model variables and parameters.

The symbol used to represent lifetime egg production should be lower case phi, as shown in on-line HTML text and tables, not the symbol shown in the pdf, which should be used only to represent the period of time spent finding an oviposition site and laying. In addition, although a number of different typefaces have been used, please assume that a given case of a given Arabic letter represents the same item in all tables and equations. read full comment

Comment on: Lynch et al. Malaria Journal, 11:383

Figure 5 Legend (John Renschler, 19 March 2013)

I think the colors described in the Figure 5 legend are backwards.

As written the red line represents halved drug pressure and the blue line is the baseline waiting time. If that were true then the panels show that Time to Emergence is shorter for the halved drug pressure than for baseline. read full comment

Comment on: Smith et al. Malaria Journal, 9:217

A pointer to previous work on this topic. (Ian Hastings, 26 November 2012)

Readers should be aware that we published a very similar PK/PD methodology and analysis of antimalarial drugs last year in AAC that is not cited or discussed in this manuscript... read full comment

Comment on: Zaloumis et al. Malaria Journal, 11:303

Pyrosequencing primers (Dylan Pillai, 26 November 2012)

We thank Woodrow et al for their important commentary [1]. Analysis of the pyrosequencing primers used for the detection of the two mutations in question (pfatp6 A623E and S769N), in this and a previous publication [2], confirmed the following. The Genbank ID (EF564342), which has also been reported by others [3], was used in the design of the pyrosequencing primers. However, this sequence is missing 14 amino acids at the N-terminus. As such, though the primers were designed to target the correct amino acid positions, they were based on the incomplete coding sequence and therefore did not identify the mutations in question. Hence, further Sanger-based sequencing will be required to confirm the presence or absence of mutations in PfATP6 sequences of these clinical isolates in relation to... read full comment

Comment on: Pillai et al. Malaria Journal, 11:131

Confusing nomenclature (Konrad Koehler, 16 July 2012)

I applaud the authors for exploring this very interesting and worthwhile approach to developing new... read full comment

Comment on: Willcox et al. Malaria Journal, 10:S8

The address of the interim report of this study (Ahmad Raeisi, 14 June 2012)

The authors wish to point out that an interim report of this study, including the data from the Hormozgan site, had been published a few weeks before this paper in the Iranian Journal of Parasitology:

Absence of asymptomatic malaria infection in endemic area of Bashagard district, Hormozgan Province in Iran; Turki H, Zoghi S, Mehrizi AA, Zakeri S, 7(1), 32-35 read full comment

Comment on: Zoghi et al. Malaria Journal, 11:126

Re: Ishag Adam (Agnieszka Kempinska-Podhorodecka, 14 June 2012)

Thank you for your interest in our paper. We would like to explain that our research was carried out during a scientific expedition organized by the Museum of Archaeology in Gda¿sk (MAG) (Poland). Since 1996, MAG has had a long-term concession for surface and excavation works in the region of the 4th Cataract on the right bank of the Nile, granted by the National Corporation for Antiquities and Museums (NCAM) in Khartoum. The expedition was led by the director of MAG, Henryk Paner. Our research is a part of a large-scale archaeological inventorisation of the middle Nile region, initiated by NCAM because of the intended dam erection in the area of Umm Duwemi village and Merowe Island. The aim of the campaign was to rescue cultural heritage and protect monuments in the areas to be flooded... read full comment

Comment on: Kempińska-Podhorodecka et al. Malaria Journal, 11:115

An alternative cellulose powder to CF11 (Bruce Russell, 14 June 2012)

Malaria groups at A*STAR and NUS (Singapore), Eijkman Institute for Molecular Biology(Indonesia) and SMRU (Thailand) have effectively substituted the use of CF11 with Sigma medium fiber Powder (cat # c6288) since September 2011. Preparation of the filter columns using the Sigma cellulose powder are as per Sriprawat et al 2009 ( . read full comment

Comment on: Venkatesan et al. Malaria Journal, 11:41

GMP artesunate (Arjen M Dondorp, 11 May 2012)

I enjoyed reading this interesting study. I just wanted to comment on the statement made by the authors regarding the Guilin artesunate product that WHO prequalification "is not the same as GMP certification". It is a prerequisite that all WHO prequalified products are manufactured to GMP and verified as such by inspectors from WHO or a stringent authority. read full comment

Comment on: Kreeftmeijer-Vegter et al. Malaria Journal, 11:102

Re Analysis for genotyping Duffy blood group in inhabitants of Sudan, the Fourth Cataract of the Nile (Ishag Adam, 11 May 2012)

I read this article with great interest and I have to congratulate the authors.
However, It is not obvious is the methods how they approached the patients and how the patients were consented. It is var difficult to convey such study in Sudan with Polish scientists without one of the local researchers. Importantly in the ethics section authors mentioned that they received ethical clearance from Poland, we do respect this but is it valid to have an ethical clearance from Poland and fostered the study in Sudan?
I hope authors would to be able to clarify these points
Best Regards read full comment

Comment on: Kempińska-Podhorodecka et al. Malaria Journal, 11:115

Update on CF11 availability (Christopher Plowe, 11 May 2012)

Unfortunately, as this paper went to press the manufacturer of CF11 put column production on hold, and subsequently suspended making them indefinitely. While they may resume in the future, we are investigating and evaluating alternative inexpensive means of leukocyte depletion. For updates, please visit the Molecular Module page on the website of the WorldWide Antimalarial Resistance Network read full comment

Comment on: Venkatesan et al. Malaria Journal, 11:41

the title (NAGUMO WALTER-RODNEY, 11 May 2012)

i think the title should have stated what phase of the trial is the study read full comment

Comment on: Adjei et al. Malaria Journal, 7:127

Additional IRS costing data (Michael White, 11 May 2012)

Readers wishing to obtain more up to date data on the costs of indoor residual spray programmes may want to check out the following excellent report from the President's Malaria Initiative:

An economic analysis of the costs of indoor residual spraying in 12 PMI countries, 2008¿2010

Prepared by
Jeffrey Sine, Rajeev Colaco, and Hannah Frawley

Michael White read full comment

Comment on: White et al. Malaria Journal, 10:337

Erratum (John Frean, 11 May 2012)

Table 1 legend should read:
Data shown are number of parasites and white blood cells (WC) counted per slide, as well as the white blood cell count (WCC, as leukocytes per µl) used to calculate the parasite density for each slide by manual, digital, and conventional methods. read full comment

Comment on: Frean Malaria Journal, 8:218

Survival rate formula assumes stable age structure (Olivier Johan Tavai Briët, 18 October 2011)

The authors use Davidson's formula (1954) to estimate the survival rate, which assumes that the mosquito population age structure is stable over the period of data collection. Since the data were collected for 10 nights in (only) one month, it is difficult to establish whether the population was approximately stable. If, for instance, the population was growing during the month surveyed, this would lead to underestimation of the survival rate. Even though there is little seasonality of rainfall in the area, the reported low survival rates should be interpreted with caution if there is no evidence that the population was stable.

Davidson, G. (1954) Estimation of the survival-rate of anopheline mosquitoes in nature. Nature, UK, 174, 792-793. read full comment

Comment on: Bugoro et al. Malaria Journal, 10:287

Correction of typo in additional file (Lisa White, 18 October 2011)

The equation lambda=R_0*((1/L)+(1/d_treat))*(I_1+I_2)/N should be lambda=R_0*((1/L)+(1/d_in))*(I_1+I_2)/N. read full comment

Comment on: White et al. Malaria Journal, 8:212

Possible effects of MDA (Dan Wolf Meyrowitsch, 18 October 2011)

Dear Dr.... read full comment

Comment on: Meyrowitsch et al. Malaria Journal, 10:188

A possible explanation for the decline: ivermectin from MDAs for LF (Edward Walker, 29 August 2011)

This interesting paper reveals a long term, downward trend in relative densities of Anopheles gambiae s.l. and Anopheles funestus in a region of northeastern Tanzania where vector control measures specifically focusing on malaria control have not previously been implemented intensively. The authors conclude that the decline is therefore not due to any specific chemical intervention against the malaria vector populations and must have some other cause.

Ironically, this same research team is studying control of lymphatic filariasis at the same study sites, and have elsewhere documented significant declines in prevalence of microfilaremia, due to Wuchereria bancrofti infection, through well done longitudinal studies. Importantly, that process has been effected by mass distribution... read full comment

Comment on: Meyrowitsch et al. Malaria Journal, 10:188

Minor error in Table 1: number of Dd2 rif genes (Alex Rowe, 29 August 2011)

The number of rif genes in parasite strain Dd2 in Table 1 should read:
rif genes 95
rif pseudogenes 24
rif truncated genes 13
Total 132 read full comment

Comment on: Claessens et al. Malaria Journal, 10:180

Could mosquitoes be dying from malaria? (Frank Ringsted, 29 August 2011)

The observed decline in malaria transmission and vector population occurs simultaneously with the spread of SP drug resistance. Could it be considered if the changes in the genetic profile of the parasite has become maladaptive for the parasite vector symbiosis, with altered parasite virulence eventually extinguishing the vector? read full comment

Comment on: Meyrowitsch et al. Malaria Journal, 10:188

Erratum Fig 4 Legend (John Hyde, 12 July 2011)

Erratum: Figure 4 legend. The description of (D) should read:

(D) Mitotic schizont developmentally a little later than (C) showing an apicoplast in the early stages of ramification. The area of intense PfSHMTc fluorescence follows the ‘Y’ shape of the apicoplast closely read full comment

Comment on: Read et al. Malaria Journal, 9:351

Omission from article (Rune Bosselmann, 06 July 2011)

The authors fail to fully describe the product they are investigating but only state insecticide content for the sides of Permanet 3.0 and not the roof, which is the interesting part of the product as it contains the combination with PBO.

The sides of Permanet 3.0 contain 2.8g/kg and the roof contains 4 g/kg (so almost 50%more) and 25 g/kg PBO. In comparison Permanet 2.0 has about 2g/kg or only half that of the roof of Permanet 3.0.

In the discussion section of the article (p. 18 in the pdf) the authors note that;

"The high dose of deltamethrin alone in the side panels of unwashed PermaNet® 3.0 could explain the significant difference
in mortality of resistant An. gambiae s.s with this net compared to the other treatment arms"

It is a... read full comment

Comment on: Koudou et al. Malaria Journal, 10:172