Figure 5.

A step by step protocol applied on day 6 post infection for predicting CM. Item₆ and Item+P₆ scores should be used estimate the probability of CM development on any time between days 7 - 10 post infection. Item⁴⁸ and Item+P⁴⁸ should be used to estimate the probability of CM development on day 8 post infection (48 hours after day 6 post infection). SHIRPA tests and its respective weights compounding each score are shown in the third line. Values obtained for each SHIRPA test should be multiplied by its respective weight and summed up to get a final score. A final score above or equal (≥) this value is considered positive and is associated with a high probability of cerebral malaria (CM) development. On the other hand, a final score under this value (<) is considered negative and is associated with a low probability of CM. U = Urination; RRa = Respiration Rate; TA = Transfer arousal; LA = Locomotor Activity; RIA = Rearing in Arena; PC = Palpebral Closure; Pi = Piloerection; PE = Pelvic Elevation; TE = Tail Elevation; PP = Positional Passivity; TC = Trunk Curl; LG = Limb Grasping; GS = Grip Strength; BT = Body Tone; CR = Corneal Reflex; TP = Toe Pinch; WM = Wire Manoeuvre; SC = Skin colour; HR = Heart Rate; LT = Limb Tone; AT = Abdominal Tone; PB = Provoked Biting; RR = Righting Reflex; CRR = Contact Righting Reflex; NG = Negative Geotaxis; F = Fear; A = Aggression; V = Vocalization; Cons = constant; P = Parasitemia.