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The infectivity of the entomopathogenic fungus Beauveria bassiana to insecticide-resistant and susceptible Anopheles arabiensis mosquitoes at two different temperatures

Christophe K Kikankie123, Basil D Brooke23, Bart GJ Knols34, Lizette L Koekemoer23, Marit Farenhorst4, Richard H Hunt12, Matthew B Thomas35 and Maureen Coetzee23*

Author Affiliations

1 School of Animal, Plant and Environmental Sciences, University of the Witwatersrand, Private Bag 3, Wits 2050, Johannesburg, South Africa

2 Vector Control Reference Unit, National Institute for Communicable Diseases of the National Health Laboratory Service, Private Bag X4, Sandringham 2131, Johannesburg, South Africa

3 Malaria Entomology Research Unit, School of Pathology, University of the Witwatersrand and the National Health Laboratory Service, Johannesburg, South Africa

4 Laboratory of Entomology, Wageningen University and Research Centre, PO Box 8031, 6700 EH, Wageningen, the Netherlands

5 Centre for Infectious Disease Dynamics and Department of Entomology, Pennsylvania State University, University Park 16802, PA, USA

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Malaria Journal 2010, 9:71  doi:10.1186/1475-2875-9-71

Published: 8 March 2010

Abstract

Background

Control of the major African malaria vector species continues to rely extensively on the application of residual insecticides through indoor house spraying or bed net impregnation. Insecticide resistance is undermining the sustainability of these control strategies. Alternatives to the currently available conventional chemical insecticides are, therefore, urgently needed. Use of fungal pathogens as biopesticides is one such possibility. However, one of the challenges to the approach is the potential influence of varied environmental conditions and target species that could affect the efficacy of a biological 'active ingredient'. An initial investigation into this was carried out to assess the susceptibility of insecticide-susceptible and resistant laboratory strains and wild-collected Anopheles arabiensis mosquitoes to infection with the fungus Beauveria bassiana under two different laboratory temperature regimes.

Methods

Insecticide susceptibility to all four classes of insecticides recommended by WHO for vector control was tested on laboratory and wild-caught An. arabiensis, using standard WHO bioassay protocols. Mosquito susceptibility to fungus infection was tested using dry spores of B. bassiana under two temperature regimes (21 ± 1°C or 25 ± 2°C) representative of indoor conditions observed in western Kenya. Cox regression analysis was used to assess the effect of fungal infection on mosquito survival and the effect of insecticide resistance status and temperature on mortality rates following fungus infection.

Results

Survival data showed no relationship between insecticide susceptibility and susceptibility to B. bassiana. All tested colonies showed complete susceptibility to fungal infection despite some showing high resistance levels to chemical insecticides. There was, however, a difference in fungus-induced mortality rates between temperature treatments with virulence significantly higher at 25°C than 21°C. Even so, because malaria parasite development is also known to slow as temperatures fall, expected reductions in malaria transmission potential due to fungal infection under the cooler conditions would still be high.

Conclusions

These results provide evidence that the entomopathogenic fungus B. bassiana has potential for use as an alternative vector control tool against insecticide-resistant mosquitoes under conditions typical of indoor resting environments. Nonetheless, the observed variation in effective virulence reveals the need for further study to optimize selection of isolates, dose and use strategy in different eco-epidemiological settings.