Table 2

Prevalence of pfcrt, pfmdr1, pfdhfr and pfdhps haplotypes in Swaziland: 1999 and 2007.

Gene

n

Genotype/Haplotype

Prevalence

1999

2007

pfcrt

Amino acids:

72 - 76

64 (1999)

CVMNK

wild-type

5 (8%)

1 (17%)

6 (2007)

CVIET

mutant

45 (70%)

5 (83%)

CVMNK/CVIET

mixed

14 (22%)

0 (0%)

pfmdr1

Amino acids:

86,184,103, 1042, 1246.

51 (1999)

86N

wild-type

12 (24%)

4 (67%)

6 (2007)

86Y

mutant

37 (73%)

2 (33%)

86F

mutant

2 (4%)

0 (0%)

50 (1999)

184Y

wild-type

39 (78%)

2 (33%)

6 (2007)

184F

mutant

11 (22%)

4 (67%)

51 (1999)

1246D

wild-type

25 (51%)

6 (100%)

6 (2007)

1246Y

mutant

25 (49%)

0 (0%)

50 (1999)

NYD

wild-type

2 (4%)

0 (0%)

6 (2007)

NFD

single-mutant 1

4 (8%)

4 (67%)

FYD

single-mutant 2

2 (4%)

0 (0%)

NYY

single-mutant 3

1 (2%)

0 (0%)

YYD

single-mutant 4

15(30%)

2 (33%)

YYY

double-mutant 1

19 (38%)

0 (0%)

NFY

double-mutant 2

4(8%)

0(0%)

YFD

double-mutant 3

2(4%)

0 (0%)

YFY

triple-mutant

1 (2%)

0 (0%)

pfdhfr

Amino acids:

50,51,59,108,164

34 (1999)

CNCSI

wild-type

3 (8%)

1 (17%)

6 (2007)

CNCSI/CIRNI

mixed

0 (0%)

1 (17%)

CIRNI

triple-mutant

28 (82%)

3 (50%)

CIRNI/CIRNL

mixed

0 (0%)

1 (17%)

CIRNI/CICNI

mixed

3 (10%)

0 (0%)

Pfdhps

Amino acids:

436, 437, 540, 581,613.

27 (1999)

SAKAA

wild-type

25 (93%)

6 (100%)

6 (2007)

SGEAA

double-mutant

2 (7%)

0 (0%)

pfdhr & pfdhps

23 (1999)

CNCSI/SAKAA

3 (13%)

1 (17%)

6 (2007)

CIRNI/SAKAA

18 (78%)

5(83%)

CIRNI/SGEAA

quintuple-mutant

2 (9%)

0 (0%)

NB: CIRNI/CIRNL, CIRNI/CICNI and CNCSI/CIRNI mixed haplotypes have been grouped into CIRNI for analysis of the prevalence of combined dhfr/dhps mutant haplotypes. n = number of successfully genotyped samples. Mutant alleles are in bold for ease of identification.

Dlamini et al. Malaria Journal 2010 9:68   doi:10.1186/1475-2875-9-68