Figure 8.

P. yoelii-infected red blood cells inhibit maturation of dendritic cells induced by stimulation of different Toll-like Receptors. To address the effect of P. yoelii-infected RBC (iRBC) on DC maturation induced by activation of different TLRs, DC were pre-incubated for 24 h with no RBC(white bars), uninfected RBC(gray bars) or iRBC(black bars) at both 10:1(solid bars) and 30:1(striped bars) ratios, and then pulsed with the following ligands of TLRs for an additional 18 hours: LPS 1 μg/ml, CpG-ODN 1 μg/ml, Zymosan 50 μg/ml, PolyI:C 25 μg/ml, flagellin 0.1 μg/ml and loxoribin 0.5 μM. DC were then analyzed by flow cytometry for expression of CD86. Data correspond to the average of triplicate samples ± standard deviation and figures are representative of one out of three independent experiments.