Table 1 |
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|
Cell proliferation assay following recovery from infection. |
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|
Spleen cell source |
Stimulant |
Amount (μg) |
Absorbance |
|
|
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|
Normal |
MSP-119 |
10 |
0.090 |
|
Normal |
MSP-119 |
20 |
0.072 |
|
F5+B6 recovered |
Con A |
10 |
0.302 |
|
F5+B6 recovered |
Con A |
20 |
0.329 |
|
F5+B6 recovered |
MSP-119 |
10 |
0.338 |
|
F5+B6 recovered |
MSP-119 |
20 |
0.445 |
|
|
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|
Normal spleen corresponds to cells from naïve mice as negative control and cells stimulated with Con A are a positive control of proliferation/viability, detected in the MTS assay. A blank value of 0.147, obtained with normal spleen cells without stimulation with MSP-119 or ConA, was subtracted from each absorbance value. Each absorbance value represents the mean from three independent experiments. |
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|
Eslava et al. Malaria Journal 2010 9:46 doi:10.1186/1475-2875-9-46 |
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