Figure 2.

Example of buffer substitution's effect on a two-band RDT. Cassettes of a two-band RDT run with blood of a malaria free subject and the RDT kit's dedicated buffer (1), injection water (2), saline (3), tap water (4) and when run with injection water in absence of blood sample (5). Cassettes "Pf" and "Pv" refer to Plasmodium falciparum or Plasmodium vivax positive samples run with the RDT kit's dedicated buffer. All cassettes show regular control lines, cassette 1 shows the expected result (no test line visible), cassettes 2, 3, 4 and 5 show false positive Pf-HRP-2 line, cassettes "Pf" and "Pv" show the expected results (positive for Pf-HRP-2 line or no test line visible respectively).