Figure 6.

Evaluation of the role of PMN, and of monocytes/macrophages, in the graft of infected and non-infected HuRBC. A peritoneal smear was drawn at different times post-infection in order to assess the phenotype of peritoneal phagocytes. (A) MP (black bar) and PMN (white bar) were identified by microscopy in order to determine the percentage of each subset among the total number of phagocytes counted on the smear (at least 500). (B) Percentages of MP (black bar) and PMN (white bar) having ingested at least one particle of malaria pigment. Results are the mean ± SD from 4 infected NOD/SCID mice. (C) Representative Giemsa staining of different organs smears performed 45 days post-infection from 3 different NOD/SCID mice (upper panel left and right: peritoneum; lower left panel: spleen, lower right panel: liver). Arrows indicate haemozoin loaded MP. (D) Effect of clo-lip and NIMP-R14 antibody on HuRBC grafting in NOD/SCID peripheral blood. HuRBC are injected alone (Black square) or in combination with clo-lip (plain circle) or with NIMP-R14 antibody (open circle). Black arrows represent the days of injection. Results are means ± SEM of 2 experiments (n = 6 mice per group).