Table 1 |
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|
Genotyping results of An. culicifacies samples by PCR-based assays and allelic association between L1014 and V1010 mutations as revealed by DNA sequencing |
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|
L1014 |
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|
Genotyping methods |
Genotypes |
|
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|
L/L |
L/F |
L/S |
F/S |
F/F |
S/S |
Total |
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|
|
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|
PCR-based assays |
176 (0.786) |
26 (0.116) |
18 (0.080) |
2 (0.009) |
2 (0.009) |
0 (0.000) |
224 |
||
|
|
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|
V/V |
64 |
26 |
0 |
0 |
2 |
0 |
92 |
||
|
|
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|
V/L |
0 |
0 |
17* |
2 |
0 |
0 |
19* |
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|
DNA-sequencing |
V1010 |
|
|||||||
|
L/L |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
||
|
|
|||||||||
|
Total |
64 |
26 |
17 |
2 |
2 |
0 |
111 |
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|
|
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|
Figures in parenthesis indicate genotype frequencies. Letters L, F, S and V refer to leucine, phenylalanine, serine and valine amino acids, respectively. *In two samples V1010L substitution was due to 3028G>C transversion and in rest samples due to 3028G>T transversion. |
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|
Singh et al. Malaria Journal 2010 9:146 doi:10.1186/1475-2875-9-146 |
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