Figure 3.

Fine epitope mapping of anti-VAR2CSA IgG. The fine specificity of the antibody response in inhibitory and non-inhibitory sera was analysed using a peptide array covering the extracellular part of 3D7 VAR2CSA. Inhibitory rat sera are shown with red vertical bars and non-inhibitory sera with black or blue vertical bars. IgG from three individual rats immunized with DBL1-3D7 (A) and DBL3-HB3 T1 (B) were analysed and IgG from two individual rats immunized with DBL4-FCR3 (red bars) and DBL4-3D7 (black bars) (C). B-cell epitopes are marked on the graph with horizontal bars in blue, red or orange and were mapped on a structural model of the corresponding domain based on the 3D7 sequence.