Table 2

Comparison of malaria diagnosis by microscopy and "standard" nested PCR The comparison between microscopy and "standard" PCR diagnosis is shown for samples collected in the Ping village (n = 102, Table 2A) and for samples collected in the Smach and Pahoy villages (n = 235, Table 2B)

Standard PCR

(%)

Microscopy

Neg

Pf

Pv

Pf+Pv

Pf+Pm

Pf+Po

Pf+Pv+Po

Pf+Pv+Pm

Pf+Pv+Pm+Po

Total

Neg

20

13

7

1

41

neg

(40.2)

Pf

4

20

1

10

6

1

6

1

49

si

(50.0)

Pv

1

1

2

Pf+Pv

1

2

4

1

1

1

10

mi

(9.8)

Total

25

33

4

22

7

1

1

7

2

102

neg

si

mi

(%)

(24.5)

(36.3)

(39.2)

Standard PCR

(%)

Microscopy

neg

Pf

Pv

Pm

Po

Pf+Pv

Pf+Pm

Pf+Po

Pv+Pm

Pv+Po

Pf+Pv+Po

Pf+Pv+Pm

Pf+Pv+Pm+Po

Total

Neg

61

47

10

3

2

14

4

2

2

1

1

7

2

156

neg

(66.4)

Pf

7

26

5

15

4

1

1

4

6

3

72

si

(31.5)

Pv

1

1

2

Pf+Pv

1

1

2

1

5

mi

(2.1)

Total

68

74

16

3

2

30

8

3

3

1

6

15

6

235

neg

si

mi

(%)

(28.9)

(40.4)

(30.6)

Pf/Pv/Pm/Po = P. falciparum, P. vivax, P. malariae and P. ovale; neg = negative; si = single infection; mi = mixed infection

Steenkeste et al. Malaria Journal 2009 8:86   doi:10.1186/1475-2875-8-86