Malaria Journal

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Towards high-throughput molecular detection of Plasmodium: new approaches and molecular markers

Nicolas Steenkeste1*, Sandra Incardona1, Sophy Chy1, Linda Duval2, Marie-Thérèse Ekala3, Pharath Lim1, Sean Hewitt4, Tho Sochantha5, Doung Socheat5, Christophe Rogier6, Odile Mercereau-Puijalon3, Thierry Fandeur3 and Frédéric Ariey1

Author Affiliations

1 Institut Pasteur du Cambodge, Laboratoire d'Epidémiologie Moléculaire, BP 983, Phnom Penh, Cambodia

2 Institut Pasteur, Unité de Pathogénie Virale, Paris, France

3 Institut Pasteur, Unité d'Immunologie Moléculaire des Parasites, CNRS URA 2581, Paris, France

4 European Commission National Malaria Control Program, Phnom Penh, Cambodia

5 National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia

6 Institut de Recherche Biomédicale des Armées – Antenne de Marseille/IMTSSA, Unité de Recherche en Biologie et Epidémiologie Parasitaires – Equipe «Moustiques et Maladies Emergentes » – UMR 6236 – URMITE, Marseille, France

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Malaria Journal 2009, 8:86 doi:10.1186/1475-2875-8-86

Published: 29 April 2009

Additional files

Additional File 1:

Agreement of Plasmodium falciparum detection by the three molecular methods. Std = "standard" nested PCR, dot18S = 18S rDNA based Dot blot detection, CYTB = cytochrome b based detection with SNP identification. Kappa coefficient (Kappa) is calculated between those three methods with 95% Confidence Interval (95% CI).

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