Malaria Journal

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Assessing the molecular divergence between Anopheles (Kerteszia) cruzii populations from Brazil using the timeless gene: further evidence of a species complex

Luísa DP Rona1, Carlos J Carvalho-Pinto2, Carla Gentile3,1, Edmundo C Grisard2 and Alexandre A Peixoto1*

Author Affiliations

1 Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, Rio de Janeiro 21045-900, RJ, Brazil

2 Departamento de Microbiologia e Parasitologia, CCB, Universidade Federal de Santa Catarina, Florianópolis 88040-970, SC, Brazil

3 School of Biological and Chemical Sciences, Queen Mary University of London, 327 Mile End Road, London, E1 4NS, UK

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Malaria Journal 2009, 8:60 doi:10.1186/1475-2875-8-60

Published: 9 April 2009

Additional files

Additional file 1:

Alignment of the DNA sequences of An. cruzii. Alignment of the DNA sequences from the timeless gene fragment from all populations of An. cruzii analysed. The translated amino acid sequence is shown above the alignment and the introns are presented in the darkened regions. Dots represent the identity of the first nucleotide sequence and asterisks represent the identity of all sequences. The non-synonymous changes found among the sequences are highlighted in yellow boxes. Flo: individuals from Florianópolis; Can: Cananéia; Juq: Juquitiba; Ita: Itatiaia; San: Santa Teresa; Bahia: individuals from Itaparica Island, Bahia State.

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