Table 1 |
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|
Fluorescence of PG and SGI - DNA adducts on Plasmodium infected erythrocytes at low parasitaemia after removal of haemoglobin and detergents |
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|
Fluorescence units (AU) |
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|
|
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|
SYBRGreen I® |
PicoGreen® |
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|
|
|
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|
% parasitaemia |
IRBC |
IRBC |
IRBC-WBC |
IRBC-WBC/W |
|
|
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|
5 |
15 ± 3 |
229 ± 12 |
243 ± 4 |
279 ± 8 |
|
2.5 |
9 ± 2 |
127 ± 7 |
141 ± 8 |
161 ± 7 |
|
1.3 |
6 ± 2 |
52 ± 1 |
83 ± 9 |
80 ± 4 |
|
0.6 |
5 ± 4 |
36 ± 3 |
36 ± 11 |
45 ± 2 |
|
0.3 |
ND1 |
20 ± 5 |
20 ± 8 |
31 ± 4 |
|
0.1 |
ND |
11 ± 3 |
11 ± 3 |
14 ± 2 |
|
0.05 |
ND |
5 ± 4 |
ND |
7 ± 3 |
|
|
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|
Limits of detection and quantification (parasitaemia %) |
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|
|
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|
LOD |
1.37 |
0.23 |
0.21 |
0.24 |
|
LOQ |
4.14 |
0.71 |
0.74 |
0.64 |
|
|
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IRBC = Infected red-blood cells; IRBC-WBC = Infected red-blood cells whithout white blood cells removal; IRBC-WBC/W = Infected red-blood cells after white blood cells removal; ND = not detected. |
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|
Moneriz et al. Malaria Journal 2009 8:279 doi:10.1186/1475-2875-8-279 |
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