Figure 6.

TLR-induced cytokine responses in foetal leukocytes are not influenced by parity. CBMC (2 million/ml) were stimulated with synthetic Hz (5 μg/ml), LPS (100 ng/ml), CpG-A (3 μg/ml) or PolyI:C (20 μg/ml). After 18 hours, supernatants were collected and analysed for IL-6, IL-10, IL-12, IFN-α, TNF-α and MIP-1α/CCL3 levels Cytokine level in unstimulated cells was subtracted from the values shown. P-values were calculated by Mann-Whitney U test or t test (see Subjects, Materials and Methods). The significance limit was P < 0.05. Data represent median values and percentiles for Women were segregated on the basis of parity as 41 paucigravidae (1st and 2nd pregnancy; white bars) and 18 multigravidae (≥ 3 pregnancies; striped bars).