Table 1

Primer pairs used to amplify the lsa-3 gene in clinical isolates genomic material.

PCR fragment

Primer forward (5'→3')

Primer reverse (5'→3')

PCR length ^a

5T

GTTAGAAAATGACAAATAGTAATTAC

GTGTTGTTGTTCTTGTTGAACAC

829

Repeats I/II (PCR1)

GTAAAAAGTGTTCAACAAGAAC

TTCCTCAGTTTCGATACCACC

1188

Repeats I/II (PCR2)

GAAAATATTTTGGAGGAAAGTCAAG

ACTGTCCTTTATTTCCTCAGTTTCG

1251

6.1-6.2

GGTATCGAAACTGAGGAAATAAAGG

CATAGCAGGAACATCAACATCCAC

789

C1-C2

GAAACTGTAACTGAACATGTAGAAC

CTTCAAGATCTTTTAAATCAGATAC

745

Repeats III

GATGTCGAAGAAGACAAGATCG

CTTTTCAATGCGTTTCTCTTTTTGG

855

3T

GGTGAAGACAAAGATGAAGTTATAG

GCAATTTTTTATTTTGATTTTTGCGTTC

665

^a, PCR fragment length in base pairs according to the lsa-3 nucleotide sequence in P. falciparum strain 3D7.

Prieur and Druilhe Malaria Journal 2009 8:247   doi:10.1186/1475-2875-8-247