Methodology
FlexiChip package: an universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance
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* Corresponding author: Nicolas Steenkeste nicolas@pasteur-kh.org
- † Equal contributors
1 Laboratoire d'épidémiologie moléculaire, Institut Pasteur du Cambodge, 5 bd Monivong, BP 983, Phnom Penh, Cambodia
2 Institut Pasteur, Génopole, Plate-forme Puces à ADN, F-75015 Paris, France
3 Amunix, 500 Ellis Street, Mountain View, CA 94043, USA
4 Institut Pasteur, Génopole, Plate-forme Génomique, F-75015 Paris, France
5 Immunologie Moléculaire des Parasites, Institut Pasteur de Paris, Paris, France
6 Swiss Tropical Institute, Socinstrasse 57, CH 4002 Basel, Switzerland
7 Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
8 Center for Clinical Vaccinology and Tropical Medicine, Oxford, UK
9 National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia
10 IMTSSA, Unité de Recherche en Biologie et Epidémiologie Parasitaires (URBEP), Marseille, France
Malaria Journal 2009, 8:229 doi:10.1186/1475-2875-8-229
Published: 15 October 2009Additional files
Additional file 1:
List of cZip Codes spotted on FlexiChip. Table S1: List of cZip Codes spotted on FlexiChip. *: discarded from the analysis (number 49 and 61)
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Additional file 2:
Scheme of the spotted oligonucleotides and expected hybridization. Table S2: Scheme of the spotted oligonucleotides and expected hybridization. A) Spotting pattern of cZip on FlexiChip (Neg = Spot of water; Cy3 and Cy5 = anchor oligonucleotides prelabeled with Cy3 or Cy5). B) Example of a FlexiChip within SNPs associated with parasite resistance to anti-malarial drugs used (blue and white areas correspond to the two samples that can be diagnosed at the same time; zip41 to 48 and 89 to 96 are not used on this microarray)
Format: DOC Size: 70KB Download file
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