Table 3 |
||||
|
Projected and observed restriction enzymes fragments |
||||
|
Restriction Enzyme Fragment Sizes (kb) |
||||
|
Probe: PkH_051981 |
||||
|
Projected |
Observed |
Difference |
Spans Sequence Gap(s) |
|
|
|
||||
|
BanII |
5.2 |
Yes |
None |
No |
|
EcoRV |
11.1 |
Yes |
None |
No |
|
HindIII |
9.8 |
23 |
13.2 |
1 and 2 |
|
HpaI |
8.7 |
18 |
9 |
1 |
|
NciI |
4.5 |
13 |
8.5 |
1 |
|
Probe: Exon 4 |
||||
|
Projected |
Observed |
Difference |
Spans Sequence Gap(s) |
|
|
|
||||
|
BanII |
11.7 |
22 |
10 |
2 |
|
EcoRV* |
>13.6 |
40+ |
? |
1 and 2 |
|
HindIII |
9.8 |
23 |
13.2 |
1 and 2 |
|
HpaI |
9.3 |
12 |
2.7 |
2 |
|
NciI |
4.5 |
13 |
8.5 |
1 |
|
|
||||
|
* The gDNA sequence downstream of the 205 SICAvar gene has not been resolved, thus the location of the next EcoRV site is unknown. |
||||
|
Lapp et al. Malaria Journal 2009 8:181 doi:10.1186/1475-2875-8-181 |
||||
