Immunophoretic rapid diagnostic tests as a source of immunoglobulins for estimating malaria sero-prevalence and transmission intensity

doi:10.1186/1475-2875-8-168

Malaria Journal

Volume 8

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Williams GS
Mweya C
Stewart L
Mtove G
Reyburn H
Cook J
Corran PH
Riley EM
Drakeley CJ

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Williams GS
Mweya C
Stewart L
Mtove G
Reyburn H
Cook J
Corran PH
Riley EM
Drakeley CJ
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Methodology

Immunophoretic rapid diagnostic tests as a source of immunoglobulins for estimating malaria sero-prevalence and transmission intensity

Geoffrey S Williams¹^,2 , Clement Mweya¹^,5 , Laveta Stewart¹^,2 , George Mtove³ , Hugh Reyburn¹^,2 , Jackie Cook¹ , Patrick H Corran²^,4 , Eleanor M Riley¹ and Chris J Drakeley¹^,2

¹Joint Malaria Programme, Kilimanjaro Christian Medical Centre, PO Box 2228, Moshi, Tanzania

²Department of Infectious & Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK

³Joint Malaria Programme, Teule Hospital, PO Box 81, Muheza, Tanzania

⁴Biotherapeutics Group, National Institute for Biological Standards and Control, South Mimms, Herts EN6 3QG, UK

⁵National Institute for Medical Research, Tukuyu station. Tanzania

author email corresponding author email

Malaria Journal 2009, 8:168doi:10.1186/1475-2875-8-168


Published:	22 July 2009

Abstract

Background

Sero-epidemiological methods are being developed as a tool for rapid assessment of malaria transmission intensity. Simple blood collection methods for use in field settings will make this more feasible. This paper describes validation of such a method, by analysing immunoglobulins from blood retained within immunophoretic rapid diagnostic tests (RDTs) for Plasmodium falciparum. RDTs are now widely used for the diagnosis of malaria and estimation of parasite rates, and this method represents a further use for these devices in malaria control.

Methods

Immunoglobulins eluted from RDTs, designed to detect parasite histidine rich protein-2 (HRP-2), were analysed by indirect ELISA for IgG recognizing the P. falciparum blood stage antigens merozoite surface protein-1₁₉(MSP-1₁₉) and apical membrane antigen-1 (AMA-1). Optimal storage conditions for RDTs were evaluated by comparing antibody responses from RDTs stored in dry or humid conditions at 4°C or at ambient temperature (with or without air-conditioning) for 7, 31 or 70 days. Antibody levels estimated using 3,700 RDT samples from attendees at health facilities in North-eastern Tanzania were compared with contemporaneously collected filter paper blood spots (FPBS) and used to estimate seroconversion rates.

Results

Storage of RDTs at 4°C was optimal for immunoglobulin recovery but short-term storage at ambient temperatures did not substantially affect anti-malarial IgG levels. Results from RDTs were comparable with those from FPBSs, for both antigens. RDT-generated titres tended to be slightly higher than those generated from FPBSs, possibly due to greater recovery of immunoglobulins from RDTs compared to filter paper. Importantly, however, RDT-based seroconversion rates, and hence serological estimates of malaria transmission intensity, agreed closely with those from FPBSs.

Conclusion

RDTs represent a practical option for collecting blood for sero-epidemiological surveys, with potential cost and logistical advantages over filter paper and other blood collection methods. RDT-based seroepidemiology can be incorporated into routine monitoring of malaria endemicity, providing information to supplement parasite prevalence rates and generating rapid, robust assessment of malaria transmission intensity at minimal extra cost.