Figure 4.

VAR2CSA recombinant proteins secreted by P. pastoris. 1 μg of purified P. pastoris recombinant proteins were analysed in 4–20% SDS-PAGE gels under non-reduced (A, B) or reduced (C) conditions. Proteins were stained with Gel Code Blue Reagent in (A) and (C) or were detected by Western blot with an anti-His Tag antibody (B).