Table 3 |
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|
PPP flux in trophozoites growing in oxidatively stressed normal RBCs and in G6PD-deficient RBCs |
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|
Sample |
Treatment |
PPP flux |
-fold increase |
|
|
|
||||
|
A |
Trophozoites growing in normal RBCs |
nil |
5.28 ± 0.84 n = 4 |
1 |
|
|
||||
|
B |
Trophozoites growing in oxidatively stressed normal RBCs |
low XO/X b |
37.4 ± 4.5 n = 3 |
7.1 |
|
|
||||
|
C |
Trophozoites growing in G6PDdeficient RBCs |
nil |
10.1 ± 1.3 n = 4 |
1.9 |
|
|
||||
|
PPP flux was measured in Sendai-virus treated trophozoites growing in normal RBCs (A), oxidatively stressed RBCs (B) and G6PD-deficient RBCs (C). Trophozoite stage parasites were isolated from host RBCs by Sendai-virus treatment and incubated in RPMI 1640 without NaHCO3, but containing 1 μCi/ml D- [1-14C] glucose at 5% haematocrit. After 90 min of preincubation at 37°C, PPP flux was measured by quantifying produced [14C]-CO2 as indicated (see Methods). Production of [14C]-CO2 is expressed as μmol/1010 cells/h at 37°C. Mean values ± SD of n separate experiments as indicated. Significance of experiments: A vs B; A vs C; and B vs C, p < 0.001 bLow XO/X: XO 0.1 mU/ml, see legend to Table 2. |
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|
Akide-Ndunge et al. Malaria Journal 2009 8:113 doi:10.1186/1475-2875-8-113 |
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