Additional file 3.

DCs were differentiated in vitro and pre-incubated or not with uninfected or P. yoelii-infected erythrocytes (upper panels) or isolated from infected mice at different times during blood-stage infection (lower panels). Naïve DO11.10 CD4⁺ T-cells were incubated for 12 h with LPS-stimulated DCs loaded with OVA peptide 323–339. Up-regulation of CD69 was measured in CD4⁺ T cells. This is a representative example of triplicated sample FACS analysis.

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Ocaña-Morgner et al. Malaria Journal 2008 7:88   doi:10.1186/1475-2875-7-88