Research
Interactions between dendritic cells and CD4+ T cells during Plasmodium infection
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* Corresponding author: Ana Rodriguez ana.rodriguez@nyu.edu
1 New York University School of Medicine, Department of Medical Parasitology, 341 E 25th street, New York, NY 10010, USA
2 Institute of Physiological Chemistry, Medical School, MTZ, Dresden University of Technology, Fiedlerstr. 42, 01307 Dresden, Germany
Malaria Journal 2008, 7:88 doi:10.1186/1475-2875-7-88
Published: 21 May 2008Additional files
DCs were differentiated in vitro and pre-incubated with control uninfected erythrocytes before loading with OVA peptide 323–339. Naïve DO11.10 T cells that are specific for this OVA epitope were isolated from transgenic mice and added to DCs. Movie shows prolonged interaction between DC and T cell.
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DCs were differentiated in vitro and pre-incubated with P. yoelii-infected erythrocytes before loading with OVA peptide 323–339. Naïve DO11.10 T cells that are specific for this OVA epitope were isolated from transgenic mice and added to DCs. Movie shows defective interaction between DC and T cell.
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Additional file 3:
DCs were differentiated in vitro and pre-incubated or not with uninfected or P. yoelii-infected erythrocytes (upper panels) or isolated from infected mice at different times during blood-stage infection (lower panels). Naïve DO11.10 CD4+ T-cells were incubated for 12 h with LPS-stimulated DCs loaded with OVA peptide 323–339. Up-regulation of CD69 was measured in CD4+ T cells. This is a representative example of triplicated sample FACS analysis.
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Additional file 4:
DO11.10 naïve CD4+ T-cells isolated from the spleens of transgenic mice and transferred into uninfected or P. yoelii-infected mice (10 days after infection). Mice were immunized or not with OVA 24 h after transfer of T-cells. Three days after immunization, transferred CD4+ T-cells from spleens of recipient mice were analysed by FACs. As control for FACs analysis mice infected or not that were not transferred with T cells were used. Transferred cells were identified using an antibody specific for DO11.10 TCR.
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