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Table 2
Primers for amplification and sequencing of Pvdhfr-ts and flanking, using PCR product B (amplifies the region from -3258 bp upstream to -420 bp upstream of P. vivax dhfr-ts). All sequences are written 5' to 3'
Primer name	Use	Sequence

S1238	PCR 1^stnested	ATC AAG GAA GGC AGA CTC CA
S1239	PCR 1^stnested	AGC GTA CTG CCG TCG AAA TA
S1259	PCR 2^ndnested, sequencing	GCC TGG TTA CTT TTG GTG GA
S1240	PCR 2^ndnested, sequencing	AAA AAC TGA GGC CAC ATT CG
S1241	sequencing	ACT TCT CTC CTG GGC AGA CTT
S1242	sequencing	GAG AGT TGG TAA TGC GGG G
S1243	sequencing	CAT GGC TGG GGA AGG CTC
S1244	sequencing	CCC TTA ACC CGC ATG CAC
S1245	sequencing	CTC CCC CCA TGG GAC AAA AA
S1246	sequencing	TTT GAT TTG ATT TGA TTT GAT TTG A
S1247	sequencing	ATG CCA CAG GGA AGT TAC AG
S1248	sequencing	CAT TTT TCA CAT TTT GGA AA
S1249	sequencing	CCT CGC GCG GGG GGG AAA
S1250	sequencing	TGC TGC AAT GCA AGT GGG T

Hawkins et al. Malaria Journal 2008 7:72 doi:10.1186/1475-2875-7-72

Table 2