Figure 4.

Migration of PBMC towards iRBC^ST -stimulated ST cells. Primary ST was stimulated with iRBC^ST, uRBC or left unstimulated for 12 hours. PBMCs added to the upper chamber of a dual chamber were allowed to migrate for 12 hours. Calcein AM-stained cells in 8 random fields for each stimulant were counted. The mean ± SD of migrated cells upon stimulation was compared to the spontaneous migration towards unstimulated ST (MED). Interaction with iRBC^ST led to a significant migration of cells towards iRBC^ST-stimulated ST compare to both controls (* = P < 0.0040). Representative data from one of two experiments is shown.