Table 1 |
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|
PCR Programme for the genes pfmdr1 86, pfcrt 76, pfdhps 540, pfdhfr 59, primer and product size |
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| Gene |
Primers |
PCR Programme |
Product size (bp) |
||
|
|
|||||
| Pfmdr1 86 |
754N 754R |
Initial step |
92°C |
3 min |
321 |
| Denaturation (35×) |
92°C |
1 min |
|||
| Annealing (35×) |
51°C |
30 s |
|||
| Extension (35×) |
72°C |
1 min |
|||
| Final step |
72°C |
3 min |
|||
| Pfcrt 76 1° nested |
76o1F N1R |
Initial step |
94°C |
3 min |
528 |
| Denaturation (40×) |
94°C |
45 s |
|||
| Annealing (40×) |
55°C |
45 s |
|||
| Extension (40×) |
72°C |
45 s |
|||
| Final step |
72°C |
3 min |
|||
| Pfcrt 76 2° nested |
76 N2F 76 N2R |
Initial step |
94°C |
3 min |
271 |
| Denaturation (35×) |
94°C |
45 s |
|||
| Annealing (35×) |
53°C |
45 s |
|||
| Extension (35×) |
72°C |
45 s |
|||
| Final step |
72°C |
3 min |
|||
| Pfdhps 540 |
540 F 540 R |
Initial step |
94°C |
3 min |
439 |
| Denaturation (40×) |
94°C |
1 min |
|||
| Annealing (40×) |
45°C |
1 min |
|||
| Extension (40×) |
72°C |
1 min |
|||
| Final step |
72°C |
3 min |
|||
| Pfdhfr 59 |
59 F 59 R |
Initial step |
94°C |
3 min |
326 |
| Denaturation (35×) |
94°C |
1 min |
|||
| Annealing (35×) |
55°C |
1 min |
|||
| Extension (35×) |
72°C |
1 min |
|||
| Final step |
72°C |
3 min |
|||
|
|
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|
Figueiredo et al. Malaria Journal 2008 7:236 doi:10.1186/1475-2875-7-236 |
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