Additional file 5.

Quantification of parasite DNA. Parasite quantification using the PF07_0076 parasite gene is not affected by human DNA within human to parasite mixtures from 1:1 down to 1:1000. The amount of HB3 input DNA in nanograms (ng) is indicated on the X-axis and the CT or threshold cycle is indicated on the Y-axis. The quantification done in the presence of either no human DNA (red diamonds), 1 ng of human DNA (yellow squares), or 10 ng of human DNA (green triangles) shows no difference when plotted.

Format: PDF Size: 12KB Download file

This file can be viewed with: Adobe Acrobat Reader

Daniels et al. Malaria Journal 2008 7:223   doi:10.1186/1475-2875-7-223