TaqMan assays discriminate between the major and minor allele. Two representative assays (corresponding to 07_000490877 and 10_001403751, with the remaining assays found in Additional Files 5 and 6) run for a subset of the parasites (corresponding to a typical running of the assay) are shown, indicating the clear separation between the signal derived from the major and minor allele. The major allele (Allele X) is displayed on the X axis and the minor allele (Allele Y) on the Y axis for 20 independent strains along with a non template control (NTC) containing only water.
Daniels et al. Malaria Journal 2008 7:223 doi:10.1186/1475-2875-7-223