Table 1

Variance components of sperm and wing length in A. gambiae and A. stephensi males.

Experiment 1: A. gambiae sperm length (μm)
Comp
df
SS
MS
V (μm2)
V (%)
F
P

male
24
3636431
151518
1012
10.7
4.98
< 0.001
testis
25
760304
30412
365
3.8
2.97
< 0.001
field
229
2345723
10243
206
2.2
1.30
0.002
sperm
2666
21031527
7889
7906
83.3


total
2944
27773985

9489
100.0










Experiment 1: A. stephensi sperm length (μm)
Comp
df
SS
MS
V (μm2)
V (%)
F
P

male
16
1530523
95658
703
10.9
4.12
0.003
testis
17
394867
23227
275
4.3
3.05
< 0.001
field
178
1355025
7612
251
3.9
1.46
< 0.001
sperm
1854
9682493
5222
5221
80.9


total
2065
12962908

6451
100.0










Experiment 2: A. gambiae wing length (mm)
Comp
df
SS
MS
V (μm2)
V (%)
F
P

family
31
0.58963
0.01902
3160.6
32.7
3.97
< 0.001
plate
32
0.153325
0.004791
0.0
0.0
0.65
0.911
male
64
0.45960
0.00741
6508.5
67.3


total
127
1.202555

9669.1
100.0










Experiment 2: A. gambiae sperm length (μm)
Comp
df
SS
MS
V (μm2)
V (%)
F
p

family
31
1655126
53391
62
0.8
1.30
0.231
plate
32
1312648
41020
50
0.6
1.14
0.325
male
64
2308407
36069
577
7.4
4.99
< 0.001
field
512
3700502
7228
10
0.1
1.01
0.407
sperm
5760
41045231
7126
7126
91.1


total
6399
50021914

7824
100.0










Experiment 3: A. gambiae wing length (mm)
Comp
df
SS
MS
V (μm2)
V (%)
F
p

family
15
0.071471
0.004765
140.8
3.1
1.06
0.416
male
44
0.197288
0.004484
4420.9
96.9


total
59
0.268759

4561.8
100.0










Experiment 3: A. gambiae sperm length (μm)
Comp
df
SS
MS
V (μm2)
V (%)
F
p

family
15
338142
22543
0
0.0
0.84
0.634
male
45
1212059
26935
354
5.0
3.31
< 0.001
field
244
1985344
8137
150
2.1
1.23
0.012
sperm
2745
18216008
6636
6636
92.9


total
3049
21751553

7140
100.0



Experiments 1, 2, and 3: the variance in sperm length (μm) and male wing length (mm) was partitioned into various components (family, tissue culture plate, male, testis, field of view and sperm). For each component the degrees of freedom (df), sum of squares (SS), mean square (MS), the variance (V) with units of μm2 or as a percent (%), the F-statistic (F), and the statistical significance (p) are shown.

Voordouw et al. Malaria Journal 2008 7:214   doi:10.1186/1475-2875-7-214

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