Table 1 |
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|
Variance components of sperm and wing length in A. gambiae and A. stephensi males. |
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| Experiment 1: A. gambiae sperm length (μm) |
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| Comp |
df |
SS |
MS |
V (μm2) |
V (%) |
F |
P |
|
|
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| male |
24 |
3636431 |
151518 |
1012 |
10.7 |
4.98 |
< 0.001 |
| testis |
25 |
760304 |
30412 |
365 |
3.8 |
2.97 |
< 0.001 |
| field |
229 |
2345723 |
10243 |
206 |
2.2 |
1.30 |
0.002 |
| sperm |
2666 |
21031527 |
7889 |
7906 |
83.3 |
||
| total |
2944 |
27773985 |
9489 |
100.0 |
|||
| Experiment 1: A. stephensi sperm length (μm) |
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| Comp |
df |
SS |
MS |
V (μm2) |
V (%) |
F |
P |
|
|
|||||||
| male |
16 |
1530523 |
95658 |
703 |
10.9 |
4.12 |
0.003 |
| testis |
17 |
394867 |
23227 |
275 |
4.3 |
3.05 |
< 0.001 |
| field |
178 |
1355025 |
7612 |
251 |
3.9 |
1.46 |
< 0.001 |
| sperm |
1854 |
9682493 |
5222 |
5221 |
80.9 |
||
| total |
2065 |
12962908 |
6451 |
100.0 |
|||
| Experiment 2: A. gambiae wing length (mm) |
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| Comp |
df |
SS |
MS |
V (μm2) |
V (%) |
F |
P |
|
|
|||||||
| family |
31 |
0.58963 |
0.01902 |
3160.6 |
32.7 |
3.97 |
< 0.001 |
| plate |
32 |
0.153325 |
0.004791 |
0.0 |
0.0 |
0.65 |
0.911 |
| male |
64 |
0.45960 |
0.00741 |
6508.5 |
67.3 |
||
| total |
127 |
1.202555 |
9669.1 |
100.0 |
|||
| Experiment 2: A. gambiae sperm length (μm) |
|||||||
| Comp |
df |
SS |
MS |
V (μm2) |
V (%) |
F |
p |
|
|
|||||||
| family |
31 |
1655126 |
53391 |
62 |
0.8 |
1.30 |
0.231 |
| plate |
32 |
1312648 |
41020 |
50 |
0.6 |
1.14 |
0.325 |
| male |
64 |
2308407 |
36069 |
577 |
7.4 |
4.99 |
< 0.001 |
| field |
512 |
3700502 |
7228 |
10 |
0.1 |
1.01 |
0.407 |
| sperm |
5760 |
41045231 |
7126 |
7126 |
91.1 |
||
| total |
6399 |
50021914 |
7824 |
100.0 |
|||
| Experiment 3: A. gambiae wing length (mm) |
|||||||
| Comp |
df |
SS |
MS |
V (μm2) |
V (%) |
F |
p |
|
|
|||||||
| family |
15 |
0.071471 |
0.004765 |
140.8 |
3.1 |
1.06 |
0.416 |
| male |
44 |
0.197288 |
0.004484 |
4420.9 |
96.9 |
||
| total |
59 |
0.268759 |
4561.8 |
100.0 |
|||
| Experiment 3: A. gambiae sperm length (μm) |
|||||||
| Comp |
df |
SS |
MS |
V (μm2) |
V (%) |
F |
p |
|
|
|||||||
| family |
15 |
338142 |
22543 |
0 |
0.0 |
0.84 |
0.634 |
| male |
45 |
1212059 |
26935 |
354 |
5.0 |
3.31 |
< 0.001 |
| field |
244 |
1985344 |
8137 |
150 |
2.1 |
1.23 |
0.012 |
| sperm |
2745 |
18216008 |
6636 |
6636 |
92.9 |
||
| total |
3049 |
21751553 |
7140 |
100.0 |
|||
|
|
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|
Experiments 1, 2, and 3: the variance in sperm length (μm) and male wing length (mm) was partitioned into various components (family, tissue culture plate, male, testis, field of view and sperm). For each component the degrees of freedom (df), sum of squares (SS), mean square (MS), the variance (V) with units of μm2 or as a percent (%), the F-statistic (F), and the statistical significance (p) are shown. |
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|
Voordouw et al. Malaria Journal 2008 7:214 doi:10.1186/1475-2875-7-214 |
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