Figure 2.

Reproducibility and specificity of the P. falciparum PFE1590w interactions with human apolipoproteins ApoA, ApoE and ApoB. Diploid strains expressing different combinations of PFE1590w and human apolipoprotein fragments as fusion proteins were tested for their ability to activate the HIS3 reporter gene in the presence of 3 mM 3-aminotriazole. The amino acid coordinates of the P. falciparum and human interacting protein fragments are indicated. Controls are strains carrying empty vectors encoding Gal4 BD or Gal4 AD, as well as fusions of Gal4 BD-PFE1350c (amino acids 9 to 135) and Gal4 AD-PFC0255c (amino acids 4 to 136), an interacting pair of P. falciparum proteins [12].