Heterologous expression of plasmodial proteins for structural studies and functional annotation
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* Corresponding author: Abraham I Louw braam.louw@up.ac.za
Malaria Journal 2008, 7:197 doi:10.1186/1475-2875-7-197
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BioMed Central: 3 citations
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Research
Krystelle Nganou-Makamdop, Maarten L van Roosmalen, Sandrine AL Audouy, Geert-Jan van Gemert, Kees Leenhouts, Cornelus C Hermsen, Robert W Sauerwein Malaria Journal 2012, 11:50 (20 February 2012) The authors describe the use of a multifunctional carrier system, bacteria like particles based on Lactococcus lactis that includes the incorporation of Plasmodium berghei CSP B cell and T cell fragments for the induction of protective immunity.
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Methodology
Edmilson Rui, Carmen Fernandez-Becerra, Satoru Takeo, Sergi Sanz, Marcus VG Lacerda, Takafumi Tsuboi, Hernando A del Portillo Malaria Journal 2011, 10:192 (14 July 2011) Malaria proteins are notoriously difficult to produce and a head-to-head comparison of the prokaryotic and eukaryotic cell-free expression system is beneficial to the large numbers of researchers trying to express such proteins.
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Research
Optimizing expression of the pregnancy malaria vaccine candidate, VAR2CSA in Pichia pastoris Marion Avril, Marianne J Hathaway, Megan M Cartwright, Severin O Gose, David L Narum, Joseph D Smith Malaria Journal 2009, 8:143 (29 June 2009) VAR2CSA is an unusually conserved member PfEMP1 family, which is transcriptionally upregulated in placental isolates. The molecule is the main candidate for a vaccine against pregnancy-associated malaria. This study describes the efforts to optimize the expression and domain boundaries, and looks at immunogenicity of the recombinant proteins.
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