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Table 1
Oligonucleotide primers.
Primer	Sequence (5'→ 3')^a	Target^b

P1	cgggaattcATGAATAATGATAATATTGGGAG	pfk1 full length (+)
P2	cccaagcttTCAGACTTGGTCATGCGGTTC	pfk1 full length (-)
P3	cgggaattcGTTGATAAGAAAGGAAAAATAATAGAT	pfk1 F2 (+)
P4	cccaagcttATGTACAAATCGATGTTTTGTATA	pfk1 F2 (-)
P5	ccgggaattcatTTTAATAATCATGATCATCAGAATC	pfk1 F3 (+)
P6	cccaagcttTAAACATTCTTGATCATCTTTTTCC	pfk1 F3 (-)
P7	ccgggaattctcAAAAAAGGACAAAACCATCAACTT	pfk1 F4 (+)
P8	cccaagcttCATATTTTTTTCCATATCTTTAATG	pfk1 F4 (-)
P9	cgcggatccgATGAAAAGCGGATTATTTTCTATG	pfk2 full length (+)
P10	ccggaattcTCACAATATATAAACTATATCATCGAATC	pfk2 full length (-)
P11	ccgggaattcATAATGATAATGATAATAATAATATTATA	pfk2 F3 (+)
P12	cccaagctttTTTAATTCCTTTTTATTATCAATTGT	pfk2 F3 (-)
P13	ccgggaattcGAATATCAAGAATATTTACCAAAAAC	pfk2 F4 (+)
P14	cccaagcttATTATGAATATGAATATTTGTATTTGA	pfk2 F4 (-)
P15	aaactgcagTGAAAACAGAATGCGATTAGTTCG	pfk1 KO (+)
P16	cgcggatccGATGATGCAAATGTTAATGGTAC	pfk1 KO (-)
P17	aaactgcagTTCTTATCTTATTGAACGGATCTC	pfk2 KO (+)
P18	cgcggatccTTGTATGGTATATATAGTAGATGC	pfk2 KO (-)
P19	GATAGCGATTTTTTTTACTGTCTG	hrp2 3'UTR (+)
P20	GAAATTAACCCTCACTAAAGGG	plasmid backbone
P21	aaggaaaaaagcggccgcAATGAATAATGATAATATTGGGAG	pfk1 (+)
P22	cccaagcttAGATATAATACCATCCGTTTGG	pfk1 (-)
P23	ccggaattcAATGAAAAGCGGATTATTTTCTATG	pfk2 (+)
P24	cgcggatccTTGTATGGTATATATAGTAGATGC	pfk2 (-)
P25	TTGTTGTGGTTCTACATCTCC	pfk2 (-)
P26	CACGAAGCCGCCACACATTGCC	hrp2 orf1
P27	TTTGATCTTGTTCACTATGGC	pfk1 (-)
P28	cccaagcttATTACAAAAATAATAATTGAAATTACT	pfk2 (-)
P29	GTAATACGACTCACTATAGGGC	plasmid backbone
P30	TTGCAATTCTGCTTCAGTTGG	cam (-)

^aSequences shown in upper case are gene specific. Underlined sequences indicate enzyme restriction sites. ^b+ and - indicate sense and anti-sense strand sequences, respectively.
Waller et al. Malaria Journal 2008 7:19 doi:10.1186/1475-2875-7-19

Table 1