Figure 4.

Localization of PfK2 in schizont-infected RBCs. Indirect Immunofluorescence Assays (IFAs) were performed using affinity purified rabbit anti-PfK2 F4 antibodies (green), and mouse monoclonal anti-MSP1 (red). Individual and merged PfK2 and MSP1 IFA panels are shown, in addition to the corresponding DAPI-labeled and phase images. Schizonts showed intense foci of labeling for PfK2, which appeared to correspond with the location of the developing merozoites within RBCs. In some cases, less intense PfK2 labeling was observed outside of the merozoites and in the RBC cytosol.