Table 2

MSP2 allele patency by lane loading volume.

MSP2 Locus
Lane loading volume




13 ul (B)
20 ul
30 ul

3D7/IC
13 μl (A)
+
-
+
-
+
-

+
79
1
74
6
73
7

-
1
12
1
1
0
2

* P

1.000

0.125

0.016


FC27
13 μl (A)
+
-
+
-
+
-

+
84
2
71
14
65
21

-
1
1
1
0
1
0

* P

1.000

0.001

< 0.001


* McNemar's test run for 13 μl lane (A) compared to other lanes. For both 3D7/IC and FC27, the number of alleles detected was greatest when 13 μl loading volume was used

Rouse et al. Malaria Journal 2008 7:185   doi:10.1186/1475-2875-7-185

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