Figure 3.

Detection of Plasmodium species using the newly developed TaqMan assay. In this example two or more specimens of P. falciparum (blue trace), P. vivax (green trace), P. ovale (yellow trace) and P. malariae (red trace) were tested. Part A displays the cycling of the FAM-labelled probe specific to P. falciparum. Part B displays the cycling of the VIC-labelled probe specific to P. malariae, P. ovale and P. vivax. Part C displays a dilution series of P. falciparum DNA, from left to right traces are generated from diluted DNA of the following concentrations: 1:10, 1:100, 1:200, 1:500, 1:1000, 1:2000, 1:5,000, 1:10,000 and 1:100,000.