Malaria Journal

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PCR-based detection of Plasmodium in Anopheles mosquitoes: a comparison of a new high-throughput assay with existing methods

Chris Bass1*, Dimitra Nikou2, Andrew M Blagborough3, John Vontas4, Robert E Sinden3, Martin S Williamson1 and Linda M Field1

Author Affiliations

1 Center for Sustainable Pest and Disease Management, Department of Biological Chemistry, Rothamsted Research, Harpenden, AL5 2JQ, UK

2 Vector Group, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L35QA, UK

3 Department of Biological Sciences, Imperial College, London, UK

4 Laboratory of Pesticide Science, Agricultural University of Athens, Iera Odos 75, 118 55, Votanikos, Athens, Greece

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Malaria Journal 2008, 7:177 doi:10.1186/1475-2875-7-177

Published: 15 September 2008

Additional files

Additional file 1:

Excel spreadsheet showing the full results of the trial of PCR methods for detection of P. falciparum using artificially infected An. stephensi mosquitoes. Mosquito samples that had no exposure to P. falciparum and buffer negative controls are highlighted in grey. A P. falciparum positive result is indicated by (Pf+) and a negative result by (-).

Format: XLS Size: 31KB Download file

This file can be viewed with: Microsoft Excel Viewer

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Additional file 2:

Excel spreadsheet showing the full results of the trial of PCR methods for detection of P. falciparum (Pf), P. vivax (Pv), P. malariae (Pm) and P. ovale (Po) using wild caught An. gambiae s.s., An. arabiensis and An. funestus mosquitoes.

Format: XLS Size: 89KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data