Additional File 1.

ClustalW alignment of PfM18AAP with Homo sapiens, Saccharomyces cerevisiae, and other Plasmodium homologues. The sequences, H. sapiens (Hs) (Q9ULAO), S. cerevisiae (Sc) (P38821), P. falciparum (Pf) (PFI1570c), P. chabaudi chabaudi (Pc) (PC000238.00.0), P. yoelii yoelii (Py) (PY03205), P. knowlesi (Pk) (PKH_073050), and P. vivax (Pv) (Pv087090)) were aligned using the ClustalW program. The five amino acids (blue) that bind the co-factor and the two amino acids (red) that cleave the substrate are conserved amongst all the species. An additional histidine (yellow) involved in enzymatic activity and another histidine (green) involved in quaternary structure stabilization are also marked. The 33 amino acid spectrin-binding region (orange) is only present in the P. falciparum aspartyl aminopeptidase.

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Lauterbach and Coetzer Malaria Journal 2008 7:161   doi:10.1186/1475-2875-7-161