Figure 5.

IL-1beta mRNA expression in immunopurified human adherent monocytes unfed or fed with HZ and treated or not with 15-HETE. Human CD14⁺ immunopurified monocytes were unfed, fed with HZ or treated with 15-HETE added at time 0 at 1–10 μM (final concentration). 6 h after phagocytosis or addition of 15-HETE. mRNA expression was measured by real-time quantitative RT-PCR in cell lysates and expressed as -fold variation over untreated monocytes. Mean values ± SD of three independent experiments. Data were analysed for significance by Student's t-test. Significance of differences (column numbers). Unfed(1) vs HZ-fed(2) monocytes, p < 0.01; unfed(1) vs 15-HETE 1 μM(3) treated monocytes, n.s.; unfed(1) vs 15-HETE 10 μM(4) treated monocytes, p < 0.05.