Figure 4.

IL-1beta production and MMP-9 enzyme activity (in cell supernatants) in human adherent monocytes unfed or fed with HZ and treated or not with 15-HETE. Human adherent monocytes were fed or not with HZ and treated or not with 15-HETE added at time 0 at 0.1–10 μM (final concentration). Panel A. After 3 h phagocytosis and a further incubation during 48 h (HZ-fed monocytes) or 48 h after addition of 15-HETE, IL-1beta levels were measured by ELISA in cell supernatants. Data are given as ng IL-1beta/ml supernatant (mean values ± SD of four independent experiments). Panel B. After 3 h phagocytosis and a further incubation during 48 h (HZ-fed monocytes) or 48 h after addition of 15-HETE, cell supernatants were separated by PAGE and MMP-9 enzyme activity measured by gelatin zymography and densitometric quantification (see legend to Figure 2 for details). The 83-kDa negative bands in the gel correspond to MMP-9 enzyme activity. Data are given as arbitrary densitometric units (mean values ± SD of four independent experiments). Data were analysed for significance by Student's t-test. Significance of differences (column/lane numbers). Unfed(1) vs HZ-fed(5) monocytes, p < 0.05; unfed(1) vs 15-HETE-treated (3,4,5) monocytes, p < 0.05 (Panel A) and p < 0.01 (Panel B).