Research
Acquisition of naturally occurring antibody responses to recombinant protein domains of Plasmodium falciparum erythrocyte membrane protein 1
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* Corresponding author: Claire L Mackintosh c.mackintosh@talk21.com
1 Kenya Medical Research Institute Centre for Geographic Medicine Research Coast (KEMRI-CGMRC), Kilifi District Hospital, Kilifi, Kenya
2 Molecular Parasitology Group, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK
3 Nuffield Department of Medicine, John Radcliffe Hospital, Oxford, UK
4 Department of Paediatrics, John Radcliffe Hospital, Oxford, UK
Malaria Journal 2008, 7:155 doi:10.1186/1475-2875-7-155
Published: 16 August 2008Additional files
Additional file 1:
Mean OD values for positive standards on each plate. Shown are the mean OD values obtained for pooled hyperimmune sera tested against each recombinant protein in turn. Each sera was tested in duplicate on each plate and each plate tested in full in duplicate.
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Additional file 2:
Range of OD values for 20 non-malaria exposed donors against each protein in turn. Shown are the minimum, maximum, mean and median for the OD obtained for 20 non-malaria exposed sera, tested against each recombinant protein in turn. Each plate was tested in duplicate and each sample was tested once on each plate.
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Additional file 3:
Proportion of individuals in each age group recognizing parasite lines A4-40 cycle and 3D7 and the clinical isolate P1. Sera from 140 individuals older than six months, was tested for reactivity against the parasite lines A4-40 cycle and 3D7 and the clinical isolate P1, a), b) and c) respectively, using flow cytometry. The proportion of individuals in each age category, with upper 95% confidence interval, scoring positive for antibody recognition are shown. Positivity was scored as defined in the text. The dark grey bars represent individuals resident in Chonyi and the light grey bars represent individuals resident in Ngerenya.
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