Evaluation of the genetic polymorphism of Plasmodium falciparum P126 protein (SERA or SERP) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the Brazilian Amazon

Lilian Rose Pratt-Riccio¹ , Selma Sallenave-Sales¹ , Joseli de Oliveira-Ferreira¹ , Bruno T da Silva¹ , Monick Lindenmeyer Guimarães² , Fátima Santos³ , Thatiane S de Simone⁴ , Mariza G Morgado² , Salvatore G de Simone⁴ , Maria de Fátima Ferreira-Da-Cruz¹ , Cláudio T Daniel-Ribeiro¹ , Mariano G Zalis⁵ , Daniel Camus⁶ and Dalma M Banic¹

¹Laboratório de Pesquisas em Malária, Departamento de Imunologia, Instituto Oswaldo Cruz, Fiocruz, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, Brazil

²Laboratório de AIDS e Imunologia Molecular, Departamento de Imunologia, IOC, Fiocruz, Rio de Janeiro, Brazil

³Departamento de Entomologia, LACEN, Porto Velho, Rondônia, Brazil

⁴Laboratório de Bioquímica de Proteínas e Peptídeos, IOC, Fiocruz, Brazil

⁵Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

⁶Service de Parasitologie-Mycologie, Faculte de Médecine, Lille, France

author email corresponding author email

Malaria Journal 2008, 7:144doi:10.1186/1475-2875-7-144


Published:	30 July 2008

Abstract

Background

The Plasmodium falciparum P126 protein is an asexual blood-stage malaria vaccine candidate antigen. Antibodies against P126 are able to inhibit parasite growth in vitro, and a major parasite-inhibitory epitope has been recently mapped to its 47 kDa N-terminal extremity (octamer repeat domain – OR domain). The OR domain basically consists of six octamer units, but variation in the sequence and number of repeat units may appear in different alleles. The aim of the present study was to investigate the polymorphism of P126 N-terminal region OR domain in P. falciparum isolates from two Brazilian malaria endemic areas and its impact on anti-OR naturally acquired antibodies.

Methods

The study was carried out in two villages, Candeias do Jamari (Rondonia state) and Peixoto de Azevedo (Mato Grosso state), both located in the south-western part of the Amazon region. The repetitive region of the gene encoding the P126 antigen was PCR amplified and sequenced with the di-deoxy chain termination procedure. The antibody response was evaluated by ELISA with the Nt47 synthetic peptide corresponding to the P126 OR-II domain.

Results

Only two types of OR fragments were identified in the studied areas, one of 175 bp (OR-I) and other of 199 bp (OR-II). A predominance of the OR-II fragment was observed in Candeias do Jamari whereas in Peixoto de Azevedo both fragments OR-I and OR-II were frequent as well as mixed infection (both fragments simultaneously) reported here for the first time. Comparing the DNA sequencing of OR-I and OR-II fragments, there was a high conservation among predicted amino acid sequences of the P126 N-terminal extremity. Data of immune response demonstrated that the OR domain is highly immunogenic in natural conditions of exposure and that the polymorphism of the OR domain does not apparently influence the specific immune response.

Conclusion

These findings confirm a limited genetic polymorphism of the P126 OR domain in P. falciparum isolates and that this limited genetic polymorphism does not seem to influence the development of a specific humoral immune response to P126 and its immunogenicity in the studied population.