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Table 2
Assay methodologies used by study participants
Lab No.	Nucleic Acid Extraction	Nucleic Acid Amplification	Sequence Targeted

1A	QIAamp DNA Mini Blood Kit	Qiagen Taq DNA polymerase	Small subunit rRNA^a
1B	QIAamp DNA Mini Blood Kit	TaqMan^®universal Master MIX	Conserved ATS domain of var genes
2A	QIAamp DNA Blood kit	Eppendorf Master Mix 2.5 × kit, gel based assay	18S rRNA^b
2B	QIAamp DNA Blood kit	QuantiTectProbe PCR Kit, real-time PCR	18S rRNA^b
3A	MagNA Pure LC Total Nucleic Acid Isolation Kit	LightCycler Fast Start DNA Master SYBRGreen I	18S rRNA
3B	MagNA Pure LC Total Nucleic Acid Isolation Kit	RealArt™ Malaria LC PCR Kit	18S rRNA
4	QIAamp DNA Blood Mini Kit	MBI Taq DNA polymerase	Small subunit rRNA gene^a
5	Boom et al., 1990 [20]	RNA T7 Polymerase, AMV-RT, RNAse H, bioMerieux	18S rRNA^c
6	MagNA Pure LC DNA Isolation Kit I	LightCycler FastStart DNA Master^PLUSHybprobe	18S rRNA
7	QIAamp DNA Mini Kit	Taqman 2 × Universal PCR Mastermix	β-tubulin^d
8	QIAamp DNA Mini Kit	TaqMan^®Universal Master Mix	18S rRNA^b
9	QIAamp DNA Mini Blood Kit	N/A	18S rRNA
10A	QIAamp DNA Mini Blood Kit	Bioline Taq Polymerase	18S rRNA^a
10B	QIAamp DNA Mini Blood Kit	Fast Start DNA Sybr Green Kit	18S rRNA
11A	Roche High Pure PCR Template Preparation Kit	Applera Taq Polymerase, Applied Biosystems	18S rRNA^e
11B	Roche High Pure PCR Template Preparation Kit	2 × Taqman Universal	18S rRNA^a
12	QIAamp DNA Mini Blood Kit	AmpliTaq Gold DNA Polymerase	18S rRNA^a
13	QIAamp DNA Blood/Mini Kit	artus Malaria PCR Kit	18S rRNA
14	High Pure PCR Template Preparation Kit	Applied Biosystems Taq Polymerase	18S rRNA^e

Assays performed according to the following published methods ^a [18,19], ^b[extraction method only, [20]], ^c[21], ^d[22], ^e[23], For other assays there were either no previously published methods or the information was not disclosed
Padley et al. Malaria Journal 2008 7:139 doi:10.1186/1475-2875-7-139

Table 2