Table 3

Treatment effect on four transmission outcomes:

Patient-level outcomes (gametocytaemia)

Variable
N patients

Univariate AUC
Ratio
of arithmetic
means (95% CI)
P value
N patients

Univariate OR slide-
positive gametocytaemia on
day of feeding
(95% CI)
P value

Antimalarial






CQ (non-artemisinin)
240
1
<0.00
337
1
<0.00
SP (non-artemisinin)
325
1.56 (1.27–1.93)
1
520
3.11 (2.19–4.40)
1
CQ-SP (non-artemisinin)
198
1.03 (0.81–1.32)

227
1.33 (0.86–2.06)

CQ-AS (ACT)
291
0.72 (0.57–0.91)

344
0.42 (0.28–0.62)

SP-AS3 (ACT)
187
0.62 (0.47–0.83)

581
0.50 (0.32–0.78)

AL (Coartem) (ACT)
407
0.18 (0.13–0.25)

404
0.15 (0.08–0.29)


ACT






non-artemisinin
872
1
<0.00
1201
1
<0.00
ACT
885
0.35 (0.31–0.41)
1
1329
0.20 (0.16–0.26)
1

ACT by pre-treatment gametocytaemia






day 0 slide-negative






non-artemisinin
727
1
<0.00
994
1
<0.00
ACT
745
0.23 (0.19–0.28)
1
1125
0.13 (0.10–0.17)
1
day 0 slide-positive






non-artemisinin
145
1
0.054
164
1

ACT
140
0.80 (0.64–1.00)

183
0.41 (0.25–0.67)
<0.00

ACT by parasitological treatment failure






No failure






non-artemisinin
459
1
<0.00
468
1
<0.00
ACT
605
0.31 (0.25–0.37)

615
0.28 (0.20–0.38)
1
Failure




1

non-artemisinin
269
1
<0.00
303

<0.00
ACT
218
0.51 (0.40–0.65)
1
233
0.33 (0.22–0.49)
1

Mosquito-level outcomes

Variable
N mosquito es
Adjusted OR mosquito infection (95% CI)*
P value
N mosquito es
Adjusted OR oocyst count per midgut in highest 50% of positive counts (4) (95% CI)*
P value

Antimalarial






CQ (non-artemisinin)
2194
1
0.002
2194
1
<0.00
SP (non-artemisinin)
6762
0.42 (0.20–0.87)

4079
0.25 (0.09–0.75)
1
CQ-SP (non-artemisinin)
1277
1.28 (0.43–3.85)

1146
0.12 (0.03–0.58)

CQ-AS (ACT)
974
0.28 (0.11–0.71)

974
0.25 (0.05–1.21)

SP-AS3 (ACT)
2793
0.29 (0.12–0.69)

1439
0.39 (0.09–1.8)

AL (Coartem) (ACT)
2299
0.22 (0.08–0.61)

292
-


ACT






non-artemisinin
11653
1
0.029
7419
1
0.165
ACT
6066
0.49 (0.33–0.73)

2705
0.45 (0.14–1.39)


ACT by pre-treatment gametocytaemia






day 0 slide-negative






non-artemisinin
8811
1
0.002
5700
1
0.008
ACT
4039
0.49 (0.31–0.77)

1419
0.11 (0.02–0.56)

day 0 slide-positive






non-artemisinin
2277
1
<0.00
1326
1
0.102
ACT
1870
0.30 (0.16–0.55)
1
1129
0.28 (0.06–1.29)


ACT by parasitological treatment failure






No failure






non-artemisinin
4773
1
0.003
2037
1
0.486
ACT
3496
0.46 (0.28–0.76)

740
0.49 (0.07–3.62)

Failure






non-artemisinin
3027
1
0.021
1574
1
0.294
ACT
897
0.37 (0.16–0.86)

572
0.33 (0.04–2.59)


at the patient level, the area under the curve of gametocyte density (microscopy measurement) during follow up (28 days), the presence of slide-positive gametocytaemia on the day of feeding; at the mosquito level, the presence of infection in mosquitoes, and high density infection in mosquitoes.

* adjusted for type of serum (autologous/control) used in transmission experiment

† P interaction with ACT/non-artemisinin <0.05

‡ Mosquitoes were infected only in study 6 where oocyst data was not collected, except one mosquito in study 5 which had a missing oocyst count.

Okell et al. Malaria Journal 2008 7:125   doi:10.1186/1475-2875-7-125